1887

Journal of General Virology header logo

Volume 83, Issue 11

RNA-dependent RNA polymerase complex of : analysis of the molecular structure with monoclonal antibodies Free

Abstract

Viral RNA-dependent RNA polymerase (RdRp) plays crucial roles in the genomic replication and subgenomic transcription of (BMV), a positive-stranded RNA plant virus. BMV RdRp is a complex of virus-encoded 1a and 2a proteins and some cellular factors, and associates with the endoplasmic reticulum at an infection-specific structure in the cytoplasm of host cells. In this study, we investigate the gross structure of the active BMV RdRp complex using monoclonal antibodies raised against the 1a and 2a proteins. Immunoprecipitation experiments showed that the intermediate region between the N-terminal methyltransferase-like domain and the C-terminal helicase-like domain of 1a protein, and the N terminus region of 2a protein are exposed on the surface of the solubilized RdRp complex. Inhibition assays for membrane-bound RdRp suggested that the intermediate region between the methyltransferase-like and the helicase-like domains of 1a protein is located at the border of the region buried within a membrane structure or with membrane-associated material.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-11-2879
2002-11-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/11/0832879a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-11-2879&mimeType=html&fmt=ahah

References

  1. Ago H., Adachi T., Yoshida A., Yamamoto M., Habuka N., Yatsunami K., Miyano M. 1999; Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus. Structure 7:1417–1426
     [Google Scholar]
  2. Ahlquist P. 1992; Bromovirus RNA replication and transcription. Current Opinion in Genetics & Development 2:71–76
     [Google Scholar]
  3. Ahlquist P., Wu S.-X., Kaesberg P., Kao C. C., Quadt R., DeJong W., Hershberger R. 1994; Protein-protein interactions and glycerophospholipids in bromovirus and nodavirus RNA replication. Archives of Virology supplementum. 9135–145
  4. Ahola T., Ahlquist P. 1999; Putative RNA capping activities encoded by brome mosaic virus: methylation and covalent binding of guanylate by replicase protein 1a. Journal of Virology 73:10061–10069
     [Google Scholar]
  5. Bressanelli S., Tomei L., Roussel A., Incitti I., Vitale R. L., Mathieu M., De Francesco R., Rey F. A. 1999; Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus. Proceedings of the National Academy of Sciences, USA 96:13034–13039
     [Google Scholar]
  6. Buck K. W. 1996; Comparison of the replication of positive-stranded RNA viruses of plants and animals. Advances in Virus Research 47:159–251
     [Google Scholar]
  7. Chen J., Ahlquist P. 2000; Brome mosaic virus polymerase-like protein 2a is directed to the endoplasmic reticulum by helicase-like viral protein 1a. Journal of Virology 74:4310–4318
     [Google Scholar]
  8. den Boon J. A., Chen J., Ahlquist P. 2001; Identification of sequences in brome mosaic virus replicase protein 1a that mediate association with endoplasmic reticulum membranes. Journal of Virology 75:12370–12381
     [Google Scholar]
  9. Dohi K., Mori M., Furusawa I., Mise K., Okuno T. 2001; Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells. Archives of Virology 146:1607–1615
     [Google Scholar]
  10. Hansen J. L., Long A. M., Schultz S. C. 1997; Structure of the RNA-dependent RNA polymerase of poliovirus. Structure 5:1109–1122
     [Google Scholar]
  11. Hardy S. F., German T. L., Loesch-Fries L. S., Hall T. C. 1979; Highly active template-specific RNA-dependent RNA polymerase from barley leaves infected with brome mosaic virus. Proceedings of the National Academy of Sciences, USA 76:4956–4960
     [Google Scholar]
  12. Horikoshi M., Nakayama M., Yamaoka N., Furusawa I., Shishiyama J. 1987; Brome mosaic virus coat protein inhibits viral RNA synthesis in vivo . Virology 158:15–19
     [Google Scholar]
  13. Horikoshi M., Mise K., Furusawa I., Shishiyama J. 1988; Immunological analysis of brome mosaic virus replicase. Journal of General Virology 69:3081–3087
     [Google Scholar]
  14. Kao C. C., Ahlquist P. 1992; Identification of the domains required for direct interaction of the helicase-like and polymerase-like RNA replication proteins of brome mosaic virus. Journal of Virology 66:7293–7302
     [Google Scholar]
  15. Kao C. C., Sivakumaran K. 2000; Brome mosaic virus, good for an RNA virologist’s basic needs. Molecular Plant Pathology 1:91–97
     [Google Scholar]
  16. Kroner P. A., Young B. M., Ahlquist P. 1990; Analysis of the role of brome mosaic virus 1a protein domains in RNA replication, using linker insertion mutagenesis. Journal of Virology 64:6110–6120
     [Google Scholar]
  17. Kuntz I. D. 1975; Protein folding. Journal of the American Chemical Society 94:4009–4012
     [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
     [Google Scholar]
  19. Lesburg C. A., Cable M. B., Ferrari E., Hong Z., Mannarino A. F., Weber P. C. 1999; Crystal structure of the RNA-dependent RNA polymerase from hepatitis C virus reveals a fully encircled active site. Nature Structural Biology 6:937–943
     [Google Scholar]
  20. Maekawa K., Furusawa I. 1984; Purification of RNA-dependent RNA polymerase from brome mosaic virus-infected barley leaves. Annals of the Phytopathological Society of Japan 50:491–499
     [Google Scholar]
  21. Miller W. A., Dreher T. W., Hall T. C. 1985; Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (−)-sense genomic RNA. Nature 313:68–70
     [Google Scholar]
  22. Mise K., Mori M., Nakayashiki H., Koyama T., Okuno T., Furusawa I. 1994; Nucleotide sequence of a set of cDNA clones derived from the brome mosaic virus ATCC66 strain and comparison with the Russian strain genome. Annals of the Phytopathological Society of Japan 60:454–462
     [Google Scholar]
  23. Mori M., Mise K., Kobayashi K., Okuno T., Furusawa I. 1991; Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 35 S RNA promoter. Journal of General Virology 72:243–246
     [Google Scholar]
  24. Nagano H., Mise K., Okuno T., Furusawa I. 1999; The cognate coat protein is required for cell-to-cell movement of a chimeric brome mosaic virus mediated by the cucumber mosaic virus movement protein. Virology 265:226–234
     [Google Scholar]
  25. Okinaka Y., Mise K., Suzuki E., Okuno T., Furusawa I. 2001; The C terminus of brome mosaic virus coat protein controls viral cell-to-cell and long-distance movement. Journal of Virology 75:5385–5390
     [Google Scholar]
  26. Okuno T., Furusawa I. 1979; RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts. Virology 99:218–225
     [Google Scholar]
  27. O’Reilly E. K., Kao C. C. 1998; Analysis of RNA-dependent RNA polymerase structure and function as guided by known polymerase structures and computer predictions of secondary structure. Virology 252:287–303
     [Google Scholar]
  28. O’Reilly E. K., Tang N., Ahlquist P., Kao C. C. 1995; Biochemical and genetic analysis of the interaction between the helicase-like and polymerase-like proteins of brome mosaic virus. Virology 214:59–71
     [Google Scholar]
  29. O’Reilly E. K., Paul J. D., Kao C. C. 1997; Analysis of the interaction of viral RNA replication proteins by using the yeast two-hybrid assay. Journal of Virology 71:7526–7532
     [Google Scholar]
  30. O’Reilly E. K., Wang Z., French R., Kao C. C. 1998; Interactions between the structural domains of the RNA replication proteins of plant-infecting RNA viruses. Journal of Virology 72:7160–7169
     [Google Scholar]
  31. Quadt R., Jaspars E. M. J. 1990; Purification and characterization of brome mosaic virus RNA-dependent RNA polymerase. Virology 178:189–194
     [Google Scholar]
  32. Quadt R., Kao C. C., Browning K., Hershberger R., Ahlquist P. 1993; Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase. Proceedings of the National Academy of Sciences, USA 90:1498–1502
     [Google Scholar]
  33. Restrepo-Hartwig M., Ahlquist P. 1996; Brome mosaic virus helicase- and polymerase-like proteins colocalize on the endoplasmic reticulum at sites of viral RNA synthesis. Journal of Virology 70:8908–8916
     [Google Scholar]
  34. Restrepo-Hartwig M., Ahlquist P. 1999; Brome mosaic virus RNA replication proteins 1a and 2a colocalize and 1a independently localizes on the yeast endoplasmic reticulum. Journal of Virology 73:10303–10309
     [Google Scholar]
  35. Rose G. D., Gierasch L. M., Smith J. A. 1985; Turns in peptides and proteins. Advances in Protein Chemistry 37:1–109
     [Google Scholar]
  36. Rost B. 1996; PHD: predicting one-dimensional protein structure by profile based neural networks. Methods in Enzymology 266:525–539
     [Google Scholar]
  37. Sacher R., Ahlquist P. 1989; Effects of deletions in the N-terminal basic arm of brome mosaic virus coat protein on packaging and systemic infection. Journal of Virology 63:4545–4552
     [Google Scholar]
  38. Schmitz I., Rao A. L. 1996; Molecular studies on bromovirus capsid protein. I. Characterization of cell-to-cell movement-defective RNA3 variants of brome mosaic virus. Virology 226:281–293
     [Google Scholar]
  39. Traynor P., Young B. M., Ahlquist P. 1991; Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread. Journal of Virology 65:2807–2815
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-11-2879
Loading
RNA-dependent RNA polymerase complex of Brome mosaic virus: analysis of the molecular structure with monoclonal antibodies
J. Gen. Virol. 83, 2879 (2002); https://doi.org/10.1099/0022-1317-83-11-2879
/content/journal/jgv/10.1099/0022-1317-83-11-2879
/content/journal/jgv/10.1099/0022-1317-83-11-2879
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error