1887

Abstract

Simian varicella virus (SVV) causes a natural varicella-like disease in nonhuman primates. Outbreaks of simian varicella occur sporadically in primate facilities. Simian varicella is used as a model for investigation of varicella-zoster virus (VZV) pathogenesis and latency. In this study, SVV gene expression and histopathology were analysed in tissues of acutely infected vervet monkeys. RT–PCR analysis demonstrated expression of specific SVV immediate early, early and late genes in the skin, lung, liver and ganglia tissues of acutely infected monkeys. Viral antigen expression and histopathology, including necrosis and inflammation, were detected in the skin, lungs, liver and spleen of infected monkeys by immunohistochemical analysis. Viral antigen expression, but little or no histopathology, was evident in the neural ganglia, the eventual site of viral latency. The study provides a foundation for further investigation on the role of viral genes in varicella pathogenesis and latency.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-4-841
2002-04-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/4/0830841a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-4-841&mimeType=html&fmt=ahah

References

  1. Allen W. P., Felsenfeld A. D., Wolf R. H., Smetana H. F. 1974; Recent studies on the isolation and characterization of delta herpesvirus. Laboratory Animal Science 24:222–228
    [Google Scholar]
  2. Ashburn C. V., Gray W. L. 2001; Characterization of the simian varicella virus glycoprotein H and L. Archives of Virology (in Press)
    [Google Scholar]
  3. Cheatham W. J., Weller T. H., Dolan T. F., Dower J. C. 1956; Varicella: report of two fatal cases with virus isolation, and serological studies. American Journal of Pathology 32:1015–1035
    [Google Scholar]
  4. Clarke P., Rabkin S. D., Inman M. V., Mahalingam R., Cohrs R., Wellish M., Gilden D. H. 1992; Molecular analysis of simian varicella virus DNA. Virology 190:597–605
    [Google Scholar]
  5. Cohen J. I., Straus S. E. 1996; Varicella-zoster virus and its replication. In Field’s Virology pp 2525–2545 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  6. Cohrs R. J., Barbour M., Gilden D. H. 1996; Varicella-zoster virus (VZV) transcription during latency in human ganglia: detection of transcripts mapping to genes 21, 29, 62, and 63 in a cDNA library enriched for VZV RNA. Journal of Virology 70:2789–2796
    [Google Scholar]
  7. Debrus S., Sadzot-Delvaux C., Nikkels A. F., Piette J., Rentier B. 1995; Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency. Journal of Virology 69:3240–3245
    [Google Scholar]
  8. Dueland A. N., Martin J. R., Devlin M. E., Wellish M., Mahalingam R., Cohrs R., Soike K. F., Gilden D. H. 1992; Acute simian varicella infection: clinical, laboratory, pathologic, and virologic features. Laboratory Investigations 66:762–773
    [Google Scholar]
  9. Fletcher T. M., Gray W. L. 1992; Simian varicella virus: Characterization of virion and infected cell polypeptides and the antigenic relatedness with varicella zoster virus. Journal of General Virology 73:1209–1215
    [Google Scholar]
  10. Gray W. L., Pumphrey C. Y., Ruyechan W. T., Fletcher T. M. 1992; The simian varicella virus and varicella zoster virus genomes are similar in size and structure. Virology 186:562–572
    [Google Scholar]
  11. Gray W. L., Williams R. L., Soike K. F. 1998a; Rapid diagnosis of simian varicella using the polymerase chain reaction. Laboratory Animal Science 48:45–49
    [Google Scholar]
  12. Gray W. L., Williams R. J., Chang R., Soike K. F. 1998b; Experimental simian varicella virus infection of St. Kitts vervet monkeys.. Journal of Medical Primatology 27:177–183
    [Google Scholar]
  13. Gray W. L., Mullis L. B., Soike K. F. 2001a; Expression of the simian varicella virus glycoprotein E. Virus Research 79:27–37
    [Google Scholar]
  14. Gray W. L., Starnes B., White M. W., Mahalingam R. 2001b; The DNA sequence of the simian varicella virus genome. Virology 284:123–130
    [Google Scholar]
  15. Inchauspe G., Nagpal S., Ostrove J. M. 1989; Mapping of two varicella-zoster virus-encoded genes that activate the expression of viral early and late genes. Virology 173:700–709
    [Google Scholar]
  16. Kinchington P. R. 1999; Latency of varicella-zoster virus: a persistently perplexing state. Frontiers in Bioscience 4:200–211
    [Google Scholar]
  17. Mahalingam R., Smith D., Wellish M., Wolf W., Dueland A. N., Cohrs R., Soike K., Gilden D. 1991; Simian varicella virus DNA in dorsal root ganglia. Proceedings of the National Academy of Sciences, USA 88:2750–2752
    [Google Scholar]
  18. Oakes J. E., d’Offay J. M. 1988; Simian varicella virus. In Virus Diseases in Laboratory and Captive Animals pp 163–174 Edited by Darai G. Boston: Martinus Nijhoff;
    [Google Scholar]
  19. Pumphrey C. Y., Gray W. L. 1992; The genomes of simian varicella virus and varicella zoster virus are colinear. Virus Research 26:255–266
    [Google Scholar]
  20. Soike K. F. 1992; Simian varicella virus infection in African and Asian monkeys: the potential for development of antivirals for animal diseases. Annals of the New York Academy of Science 653:323–333
    [Google Scholar]
  21. White T. M., Gilden D. H., Mahalingam R. 2001; An animal model of varicella-zoster virus infection. Brain Pathology 11:475–479
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-4-841
Loading
/content/journal/jgv/10.1099/0022-1317-83-4-841
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error