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Volume 97, Issue 5

Isolation and characterization of a novel type of rotavirus species A in sugar gliders () Free

Abstract

To estimate the risk of interspecies transmission of rotavirus species A (RVA) from exotic pets to other mammalian species, the prevalence of RVA in sugar gliders () was investigated. RVAs were detected in 10 of 44 sugar gliders by reverse transcription (RT)-semi-nested PCR. These viruses were classified as G27P[3] and G27P[36] genotypes, with G27 and P[36] being new genotypes as assigned by the Rotavirus Classification Working Group. To characterize sugar glider RVA in detail, one strain, RVA/SugarGlider-tc/JPN/SG385/2012/G27P[36] (SG385-tc), was isolated. All of the genes of the strain were classified as new genotypes (G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12). The enterotoxin domain in NSP4, which is important for the induction of diarrhoea, was conserved between SG385-tc and previously reported mammalian strains, suggesting the potential of sugar glider RVA to cause diarrhoea in mammalian species. In fact, seven out of nine suckling mice inoculated orally with 3.9 × 10 f.f.u. of strain SG385-tc had diarrhoea and the 50 % diarrhoea-inducing dose (DD) of strain SG385-tc in suckling mice was 1.2 × 10 f.f.u. Our findings suggest that sugar glider RVA is infective to and possibly pathogenic in other mammalian species.

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© 2016 The Authors
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2016-05-01
2024-04-27
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Isolation and characterization of a novel type of rotavirus species A in sugar gliders (Petaurus breviceps)
J. Gen. Virol. 97, 1158 (2016); https://doi.org/10.1099/jgv.0.000433
/content/journal/jgv/10.1099/jgv.0.000433
/content/journal/jgv/10.1099/jgv.0.000433
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