1887

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Volume 105, Issue 3

Incidental finding of a human-like tusavirus in a lamb with lip lesions and fatal pneumonia Open Access

Abstract

Tusaviruses in the genus of family were first identified in a diarrhoeic Tunisian child in 2014. Thereafter, high prevalence of a genetically similar virus was demonstrated in faeces from caprine and ovine species in Hungary. Here, we describe an investigation into the cause of scabby lip lesions in a 6 month-old lamb, submitted from a farm experiencing weight loss and scouring in lambs in England. Transmission electron microscopy visualised small circular particles of 18 and 22 nm in diameter in lip lesions identified as tusavirus and flumine parvovirus by Next Generation Sequencing. Liver, kidney, lung, small intestine content and faeces were also strongly positive for the tusavirus DNA as well as 10 % of faecal samples of the flock collected 2 months after the initial lip sampling. NS1 and VP1 amino acid sequences of this tusavirus displayed 99.5 and 92.89 % identity to those of a human tusavirus, respectively. These amino acid identities were at 95.5 and 89.68 % when compared to those of a goat tusavirus. Phylogenetic analysis of the NS1 and VP1 also grouped the virus in the genus and close to tusaviruses detected in human, ovine and caprine species. Wider surveillance of the virus indicated a broader geographical distribution for the virus in England. Histology of the lip tissue revealed localised areas of epidermal hyperplasia and hyperkeratosis affecting haired skin, with mild leucocyte infiltration of the subjacent dermis, but no changes to implicate virus involvement. Flumine parvovirus was concluded to be an environment contaminant. Broader studies in prevalence of these virus in UK sheep flocks and human population, animal models and experimental infections could provide insights into the pathogenesis of these novel viruses and their zoonotic potential.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): lip lesions , ovine parvovirus , pneumonia , protoparvovirus , pustular dermatitis and tusavirus
Funding
This study was supported by the:
  • Nemzeti Kutatási Fejlesztési és Innovációs Hivatal (Award NKFIH FK134311)
    • Principle Award Recipient: GáborReuter
  • Department for Environment, Food and Rural Affairs, UK Government
    • Principle Award Recipient: HannahDavies
© 2024 Crown copyright
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-03-05
2024-04-27
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Incidental finding of a human-like tusavirus in a lamb with lip lesions and fatal pneumonia
J. Gen. Virol. 105, 001968 (2024); https://doi.org/10.1099/jgv.0.001968
/content/journal/jgv/10.1099/jgv.0.001968
/content/journal/jgv/10.1099/jgv.0.001968
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