1887

Abstract

Expression of the core capsid protein P3 of in a baculovirus system resulted in the formation of single-shelled core-like particles in insect cells in the absence of any other capsid proteins. Double-shelled virus-like particles were also observed upon mixing or co-expression of P3 and the major outer capsid protein P8, suggesting that P3 and P8 have the ability to form double-shelled particles both and . Core protein P7 expressed in a similar manner was incorporated into the virus-like particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18904-0
2003-04-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/4/vir840981.html?itemId=/content/journal/jgv/10.1099/vir.0.18904-0&mimeType=html&fmt=ahah

References

  1. Hagiwara K., Kobayashi J., Tomita M., Yoshimura T. 2001; Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1. Arch Virol 146:181–187
    [Google Scholar]
  2. Kano H., Koizumi M., Noda H., Mizuno H., Tsukihara T., Ishikawa K., Hibino H., Omura T. 1990; Nucleotide sequence of rice dwarf virus (RDV) genome segment S3 coding for 114 K major core protein. Nucleic Acids Res 25:6700
    [Google Scholar]
  3. Lin N.-S. 1984; Gold–IgG complexes improve the detection and identification of viruses in leaf dip preparation. J Virol Methods 8:181–190
    [Google Scholar]
  4. Mao Z., Li J., Xu Y., Zheng H. H., Schiemann J., Casper R., Chen Z. L. 1998; The 42K protein of rice dwarf virus is a post-translational cleavage product of the 46K outer capsid protein. Arch Virol 143:1831–1838
    [Google Scholar]
  5. Nakashima K., Kakutani T., Minobe Y. 1990; Sequence analysis and product assignment of segment 7 of the rice dwarf virus genome. J Gen Virol 71:725–729
    [Google Scholar]
  6. Omura T., Ishikawa K., Hirano H., Ugaki M., Minobe Y., Tsuchizaki T., Kato H. 1989; The outer capsid protein of rice dwarf virus is encoded by genome segment S8. J Gen Virol 70:2759–2764
    [Google Scholar]
  7. Omura T., Jan J. 1999; Role of outer capsid proteins in transmission of Phytoreovirus by insect vectors. Adv Virus Res 54:15–43
    [Google Scholar]
  8. Ueda S., Uyeda I. 1997; The rice dwarf phytoreovirus structural protein P7 possesses non-specific nucleotide acids binding activity in vitro . Molecular Plant Pathology [On-Line]
    [Google Scholar]
  9. Suzuki N., Sugawara M. 1991; Outer capsid protein heterogeneity of rice dwarf phytoreovirus. J Gen Virol 72:2239–2342
    [Google Scholar]
  10. Zheng H., Yu L., Wei C., Hu D., Shen Z., Chen Z., Li Yi. 2000; Assembly of double-shelled, virus-like particles in transgenic rice plants expressing two major structural proteins of Rice dwarf virus . J Virol 74:9808–9810
    [Google Scholar]
  11. Wu S., Hammar L., Xing L., Markarian S., Yan J., Iwasaki K., Fujiyoshi Y., Omura T., Cheng H. 2000; Phytoreovirus T =1 core plays critical roles in organizing the outer capsid of T =13 quasi-equivalence. Virology 271:18–25
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18904-0
Loading
/content/journal/jgv/10.1099/vir.0.18904-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error