1887

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Volume 87, Issue 5

Biological characterization of human immunodeficiency virus type 1 subtype C protease carrying indinavir drug-resistance mutations Free

Abstract

Human immunodeficiency virus type 1 subtype C isolates belong to one of the most prevalent strains circulating worldwide and are responsible for the majority of new infections in the sub-Saharan region and other highly populated areas of the globe. In this work, the impact of drug-resistance mutations in the protease gene of subtype C viruses was analysed and compared with that of subtype B counterparts. A series of recombinant subtype C and B viruses was constructed carrying indinavir (IDV)-resistance mutations (M46V, I54V, V82A and L90M) and their susceptibility to six FDA-approved protease inhibitor compounds (amprenavir, indinavir, lopinavir, ritonavir, saquinavir and nelfinavir) was determined. A different impact of these mutations was found when nelfinavir and lopinavir were tested. The IDV drug-resistance mutations in the subtype C protease backbone were retained for a long period in culture without selective pressure when compared with those in subtype B counterparts in washout experiments.

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2006-05-01
2024-04-27
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Biological characterization of human immunodeficiency virus type 1 subtype C protease carrying indinavir drug-resistance mutations
J. Gen. Virol. 87, 1303 (2006); https://doi.org/10.1099/vir.0.81517-0
/content/journal/jgv/10.1099/vir.0.81517-0
/content/journal/jgv/10.1099/vir.0.81517-0
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