1887

Abstract

The complete nucleotide sequence of the genomic RNA 1 (8745 nt) and RNA 2 (4986 nt) of Citrus leprosis virus cytoplasmic type (CiLV-C) was determined using cloned cDNA. RNA 1 contains two open reading frames (ORFs), which correspond to 286 and 29 kDa proteins. The 286 kDa protein is a polyprotein putatively involved in virus replication, which contains four conserved domains: methyltransferase, protease, helicase and polymerase. RNA 2 contains four ORFs corresponding to 15, 61, 32 and 24 kDa proteins, respectively. The 32 kDa protein is apparently involved in cell-to-cell movement of the virus, but none of the other putative proteins exhibit any conserved domain. The 5′ regions of the two genomic RNAs contain a ‘cap’ structure and poly(A) tails were identified in the 3′-terminals. Sequence analyses and searches for structural and non-structural protein similarities revealed conserved domains with members of the genera , , and , although phylogenetic analyses strongly suggest that CiLV-C is a member of a distinct, novel virus genus and family, and definitely demonstrate that it does not belong to the family , as previously proposed. Based on these results it was proposed that Citrus leprosis virus be considered as the type member of a new genus of viruses, .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82038-0
2006-09-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/9/2721.html?itemId=/content/journal/jgv/10.1099/vir.0.82038-0&mimeType=html&fmt=ahah

References

  1. Bitancourt A. A. 1955; Estudos sobre a leprose dos citros III – transmissão natural às frutas. Arq Instit Biolog 22:205–218 (in Portuguese
    [Google Scholar]
  2. Boari A.J., Freitas-Astua J., Ferreira P. T. O., Neder D. G., Nogueira N. L., Rossi M. L., Kitajima E. W. 2004; Purification and serology of the coffee ringspot virus. Summa Phytopathol 30:453–458
    [Google Scholar]
  3. Chagas C. M., Rosseti V., Chiavegatto L. G. 1983; Effectiveness of the different life cycle stages of Brevipalpus phoenicis Geijskes in leprosis transmission. In Proceedings of the 9th IOCV Conference pp  211–214 Riverside, CA:
    [Google Scholar]
  4. Colariccio A., Lovisolo O., Chagas C. M., Galetti S. R., Rosseti V., Kitajima E. W. 1995; Mechanical transmission and ultrastructural aspects of Citrus leprosis virus. Fitopatol Bras 20:208–213
    [Google Scholar]
  5. Colariccio A., Lovisolo O., Boccardo G., Chagas C. M., D'Aquilio M., Rossetti V. 2000; Preliminary purification and double stranded RNA analysis of citrus leprosis virus. In Proceedings of the 14th IOCV Conference pp  159–163 Riverside, CA:
    [Google Scholar]
  6. Doi Y., Chang M. U., Yora K. 1977; Orchid fleck virus. CMI/AAB Descriptions of Plant Viruses no: 183 Kew, UK: Commonwealth Mycological Institute and Association of Applied Biologists; http://www.dpvweb.net/dpv/showdpv.php?dpvno=183
    [Google Scholar]
  7. Dominguez F. S., Bandel A., Childers C., Kitajima E. W. 2001; First report of citrus leprosis on Panama. Plant Dis 85:228
    [Google Scholar]
  8. Fawcett H. S. 1936 Citrus Diseases and Their Control, 2nd edn. New York & London: McGraw-Hill;
    [Google Scholar]
  9. Freitas-Astúa J., Kitajima E. W., Locali E. C., Antonioli-Luizon R., Bastianel M., Machado M. A. 2005; Further evidence to support that citrus leprosis virus-cytoplasmic and nuclear types are different viruses. In Annals of the XLV Annual Meeting of the American Phytopathological Society, Caribbean Division, Costa Rica, June 2005 p– 93
    [Google Scholar]
  10. Gibbs A., Mackenzie A. 1997; A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. J Virol Methods 63:9–16 [CrossRef]
    [Google Scholar]
  11. Jackson A. O., Dietzgen R. G., Goodin M. M., Bragg J. N., Deng M. 2005; Biology of plant rhabdoviruses. Annu Rev Phytopathol 43:623–660 [CrossRef]
    [Google Scholar]
  12. Kitajima E. W., Muller G. W., Costa A. S., Yuki W. 1972; Short rod-like particles associated with citrus leprosis. Virology 50:254–258 [CrossRef]
    [Google Scholar]
  13. Kitajima E. W., Ferreira P. T. O., Freitas-Astua J., Machado M. A. 2004; Ocorrência da leprose dos citros, tipo nuclear (CiLV-N), nos municípios paulistas de Monte Alegre do Sul e Amparo. Summa Phytopathol 30:68 (in Portuguese
    [Google Scholar]
  14. Knorr L. C. 1950; Etiological association of a Brevipalpus mite with Florida scaly bark of citrus. Phytopathology 40:15
    [Google Scholar]
  15. Knorr L. C. 1968; Studies on the etiology of leprosis in citrus. In Proceedings of the 4th IOCV Conferenc e pp  332–341 Gainesville, FL, USA:
    [Google Scholar]
  16. Kondo H., Maeda T., Tamada T. 2003; Orchid fleck virus: brevipalpus californicus mite transmission, biological properties and genome structure. Exp Appl Acarol 30:215–223 [CrossRef]
    [Google Scholar]
  17. Locali E. C., Freitas-Astua J., Souza A. A., Takita M. A., Astua-Monge G., Antonioli R., Kitajima E. W., Machado M. A. 2003; Development of a molecular tool for the diagnosis of leprosis a major treat to the citrus production in the Americas. Plant Dis 87:1317–1321 [CrossRef]
    [Google Scholar]
  18. Rodrigues J. C. V. 2000; Relações patógeno-vetor-planta no sistema leprose dos citros. Tese (Doutorado) – Centro de Energia Nuclear na Agricultura, Universidade de São Paulo Piracicaba, 168 (in Portuguese
    [Google Scholar]
  19. Rodrigues J. C. V., Nogueira N. L., Freitas D. S., Prates H. 1997; Virus-like particles associated with Brevipalpus phoenicis Geijskes (Acari: Tenuipalpidae), vector of citrus leprosis virus. An Soc Entomol Brasil 26:391–395 [CrossRef]
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Swofford D. L. 2000 Phylogenetic analysis using parsimony, version 4.0b4a Champaign, IL: Ilinois Natural History Survey;
    [Google Scholar]
  22. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  23. Valverde R. A., Nameth S. T., Jordan R. L. 1990; Analysis of double-stranded RNA for plant virus diagnostic. Plant Dis 74:255–258
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82038-0
Loading
/content/journal/jgv/10.1099/vir.0.82038-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error