1887

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Volume 88, Issue 11

Murine cytomegalovirus open reading frame augments virus replication both and Free

Abstract

Murine cytomegalovirus mutant Rc29, with a premature stop codon mutation in the open reading frame (ORF), produced no apparent phenotype in cell culture or following infection of BALB/c mice. In contrast, a similar mutant virus, Rc29.1, with a premature stop codon mutation in its ORF, showed reduced virus yields (2–3 log p.f.u. ml) in tissue culture. Mutant virus yields in BALB/c mice were delayed, reduced (∼1 log p.f.u. per tissue) and persisted less well in salivary glands compared with wild-type (wt) and revertant (Rv29.1) virus. In severe combined immunodeficiency mice, Rc29.1 virus showed delayed and reduced replication initially in all tissues (liver, spleen, kidneys, heart, lung and salivary glands). This delayed death until 31 days post-infection (p.i.) compared with wt (23 days p.i.) but at death virus yields were similar to wt. gene transcription was initiated at early times post-infection, while production of a transcript from ORF in the presence of cycloheximide indicated that it was an immediate-early gene. ORFs and are expressed from a bicistronic message, which is spliced infrequently. However, it is likely that each ORF expresses its own protein, as antiserum derived in rabbits to the m29.1 protein expressed in bacteria from the ORF detected only one protein in Western blot analysis of the size predicted for the m29.1 protein. Our results suggest that neither ORF is essential for virus replication but is important for optimal viral growth and .

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2007-11-01
2024-05-13
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Murine cytomegalovirus open reading frame m29.1 augments virus replication both in vitro and in vivo
J. Gen. Virol. 88, 2941 (2007); https://doi.org/10.1099/vir.0.83133-0
/content/journal/jgv/10.1099/vir.0.83133-0
/content/journal/jgv/10.1099/vir.0.83133-0
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