- Volume 67, Issue 10, 1986
Volume 67, Issue 10, 1986
- Plant
-
-
-
Induction by Salicylic Acid of Pathogenesis-related Proteins and Resistance to Alfalfa Mosaic Virus Infection in Various Plant Species
More LessSummarySpraying tobacco plants with salicylic acid induces both the synthesis of ‘pathogenesis-related’ (PR) proteins and resistance to viruses that can induce necrotic lesions. We show that spraying Samsun NN tobacco with salicylic acid induced the production of PR-1 mRNAs and inhibited the systemic multiplication of alfalfa mosaic virus (AlMV) by 90%. Salicylic acid treatment also induced the synthesis of PR proteins in bean and cowpea plants, and reduced by 75% the production of local lesions in AlMV-infected bean plants. Salicylic acid inhibited the replication of AlMV in cowpea protoplasts by up to 99%, depending on the mode of application. In AlMV-inoculated cowpea protoplasts, the production of viral minus-strand RNA, plus-strand RNA and coat protein was abolished, indicating that salicylic acid inhibits an early step in the AlMV replication cycle. The viability of the cells and the synthesis of host proteins were not affected by salicylic acid. Another aromatic compound, p-coumaric acid, induced neither PR proteins nor resistance to virus infection.
-
-
-
-
Antigenic Analysis of Potato Virus X by Means of Monoclonal Antibodies
R. Koenig and L. TorranceSummaryAt least three different antigenic determinants were distinguished on the capsid protein of the B strain of potato virus X by their differential reactivity with monoclonal antibodies. One determinant (or group of determinants) was located on the protruding N terminus which, in the assembled virus particles, is readily split off by proteases in crude plant sap or by trypsin. A second determinant (or group of determinants) was located outside the protruding N terminus on the surface of the undisturbed virus particles. In partially denatured preparations containing the protruding N terminus, this determinant became inaccessible. A third determinant (or group of determinants) became exposed only after some denaturation of the virus particles, e.g. when they were applied directly to ELISA plates or nitrocellulose membranes. In contrast to the other two determinants, this determinant was not destroyed by extensive denaturation, such as heating in solution with SDS and 2-mercaptoethanol.
-
-
-
Figwort Mosaic Virus DNA Replicates in Cultured Datura stramonium Cells
More LessSummaryCellular forms of the DNA of the caulimovirus figwort mosaic virus (FMV), isolated from Datura stramonium leaves and callus cells derived from FMV-infected leaf tissue, have been studied. One- and two-dimensional gel electrophoresis and blot hybridization experiments showed the presence of various truncated forms of FMV DNA reminiscent of the putative replication intermediates of cauliflower mosaic virus (CaMV) thought to be generated by a mechanism involving reverse transcription. FMV DNA was found to be qualitatively and quantitatively similar in leaf and callus tissue, suggesting that, in contrast to CaMV, FMV can multiply efficiently in actively proliferating cells cultured in vitro.
-
-
-
A Non-structural Protein of Alfalfa Mosaic Virus in the Walls of Infected Tobacco Cells
More LessSummaryThe 32000 mol. wt. (32K) non-structural protein of alfalfa mosaic virus, P3, has previously been detected in a crude membrane fraction of infected tobacco leaves, where it accumulated transiently at the beginning of the infection period. We show here, by immunoblotting with an antiserum to a synthetic peptide corresponding to the C terminus of the protein, that the majority of P3 is in the cell wall fraction where it remains throughout infection, both at 25 °C and at 10 °C. The cell wall-associated, P3-related material is heterogeneous and contains polypeptide species of slightly lower electrophoretic mobility than the major in vitro translation product of RNA 3, which suggests that P3 may be post-translationally modified.
-
-
-
The 368-Nucleotide Satellite of Cucumber Mosaic Virus Strain Y from Japan Does Not Cause Lethal Necrosis in Tomato
More LessSummaryCucumber mosaic virus (CMV) strain Y from Japan was propagated in Xanthi tobacco. Virus isolated from four successive passages was analysed for its constituent RNAs by polyacrylamide gel electrophoresis (PAGE) under semi-denaturing and fully denaturing conditions. This revealed the initial presence of the 368-nucleotide satellite RNA Y-CARNA 5 (for Y-CMV-associated RNA 5) and the subsequent emergence of a small RNA in the 335-nucleotide size range, which we termed Yn-CARNA 5. The appearance of the smaller Yn-CARNA 5 correlated positively with the increasing tomato necrosis-inducing properties of tissue extracts from the later tobacco passages. After sucrose gradient ultracentrifugation of total CMV-Y RNA, the fraction containing Y-CARNA 5 and Yn-CARNA 5 was subjected to PAGE under semi-denaturing conditions, which provided highly purified gel-eluted preparations of the two CARNA 5 species. When tested for their tomato necrosis-inducing capability in the presence of helper virus genomic RNAs, Y-CARNA 5 was shown to be incapable of eliciting lethal necrosis in Rutgers tomato whereas Yn-CARNA 5 was highly necrogenic.
-
Volumes and issues
-
Volume 105 (2024)
-
Volume 104 (2023)
-
Volume 103 (2022)
-
Volume 102 (2021)
-
Volume 101 (2020)
-
Volume 100 (2019)
-
Volume 99 (2018)
-
Volume 98 (2017)
-
Volume 97 (2016)
-
Volume 96 (2015)
-
Volume 95 (2014)
-
Volume 94 (2013)
-
Volume 93 (2012)
-
Volume 92 (2011)
-
Volume 91 (2010)
-
Volume 90 (2009)
-
Volume 89 (2008)
-
Volume 88 (2007)
-
Volume 87 (2006)
-
Volume 86 (2005)
-
Volume 85 (2004)
-
Volume 84 (2003)
-
Volume 83 (2002)
-
Volume 82 (2001)
-
Volume 81 (2000)
-
Volume 80 (1999)
-
Volume 79 (1998)
-
Volume 78 (1997)
-
Volume 77 (1996)
-
Volume 76 (1995)
-
Volume 75 (1994)
-
Volume 74 (1993)
-
Volume 73 (1992)
-
Volume 72 (1991)
-
Volume 71 (1990)
-
Volume 70 (1989)
-
Volume 69 (1988)
-
Volume 68 (1987)
-
Volume 67 (1986)
-
Volume 66 (1985)
-
Volume 65 (1984)
-
Volume 64 (1983)
-
Volume 63 (1982)
-
Volume 62 (1982)
-
Volume 61 (1982)
-
Volume 60 (1982)
-
Volume 59 (1982)
-
Volume 58 (1982)
-
Volume 57 (1981)
-
Volume 56 (1981)
-
Volume 55 (1981)
-
Volume 54 (1981)
-
Volume 53 (1981)
-
Volume 52 (1981)
-
Volume 51 (1980)
-
Volume 50 (1980)
-
Volume 49 (1980)
-
Volume 48 (1980)
-
Volume 47 (1980)
-
Volume 46 (1980)
-
Volume 45 (1979)
-
Volume 44 (1979)
-
Volume 43 (1979)
-
Volume 42 (1979)
-
Volume 41 (1978)
-
Volume 40 (1978)
-
Volume 39 (1978)
-
Volume 38 (1978)
-
Volume 37 (1977)
-
Volume 36 (1977)
-
Volume 35 (1977)
-
Volume 34 (1977)
-
Volume 33 (1976)
-
Volume 32 (1976)
-
Volume 31 (1976)
-
Volume 30 (1976)
-
Volume 29 (1975)
-
Volume 28 (1975)
-
Volume 27 (1975)
-
Volume 26 (1975)
-
Volume 25 (1974)
-
Volume 24 (1974)
-
Volume 23 (1974)
-
Volume 22 (1974)
-
Volume 21 (1973)
-
Volume 20 (1973)
-
Volume 19 (1973)
-
Volume 18 (1973)
-
Volume 17 (1972)
-
Volume 16 (1972)
-
Volume 15 (1972)
-
Volume 14 (1972)
-
Volume 13 (1971)
-
Volume 12 (1971)
-
Volume 11 (1971)
-
Volume 10 (1971)
-
Volume 9 (1970)
-
Volume 8 (1970)
-
Volume 7 (1970)
-
Volume 6 (1970)
-
Volume 5 (1969)
-
Volume 4 (1969)
-
Volume 3 (1968)
-
Volume 2 (1968)
-
Volume 1 (1967)