1887

Abstract

SUMMARY

Bacteriophage 7-7-1 is shown to adsorb specifically to the complex flagella of its host H13–3. Deflagellation of motile cells before the addition of phage leads to a complete inhibition of phage propagation for at least 60 min. Among phage-resistant mutants, many non-motile () and non-flagellated () derivatives of H13–3 have been selected. Electron microscopic observations indicate that bacteriophage 7-7-1 attaches with its short tail fibres to the conspicuous helical filament of flagella. This attachment is reversible; irreversible phage adsorption takes place at the flagellar base. It is postulated that phage 7-7-1 moves along the rotating flagellum towards a final receptor next to the insertion site of the flagellum, where tail contraction and injection of phage nucleic acid occurs.

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1977-01-01
2024-04-26
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References

  1. Barnet Y. M. 1972; Bacteriophages of Rhizobium trifolii. I. Morphology and host range. Journal of General Virology 15:1–15
    [Google Scholar]
  2. Berg C. H., Anderson R. A. 1973; Bacteria swim by rotating their flagellar filaments. Nature, London 245:380–382
    [Google Scholar]
  3. Edwards S., Meynell G. G. 1968; The widespread occurrence of enteric flagellar phages. Journal of General Virology 2:443–444
    [Google Scholar]
  4. Eiserling F. A. 1967; The structure of Bacillus subtilis bacteriophage PBS1. Journal of Ultrastructure Research 17:342–347
    [Google Scholar]
  5. Enomoto M. 1966; Genetic studies of paralyzed mutants in Salmonella. 1. Genetic fine structure of the mot loci in Salmonella typhimurium. Genetics 54:715–726
    [Google Scholar]
  6. Gabor M. 1965; Transformation of streptomycin markers in rough strains of Rhizobium lupini. II. The relation between the determinant of streptomycin dependence and those for streptomycin. Genetics 52:905–913
    [Google Scholar]
  7. Iino T., Mitani M. 1967; Infection of Serratia marcescens by bacteriophage k.. Journal of Virology 1:445–447
    [Google Scholar]
  8. Jollick J. D., Wright B. L. 1974; A flagella-specific bacteriophage for Caulobacter. Journal of General Virology 22:197–205
    [Google Scholar]
  9. Joys T. M. 1965; Correlation between susceptibility to bacteriophage PBS1 and motility in Bacillus subtilis. Journal of Bacteriology 90:1575–1577
    [Google Scholar]
  10. Lindberg A. A. 1973; Bacteriophage receptors. Annual Review of Microbiology 27:205–241
    [Google Scholar]
  11. Lotz W., Mayer F. 1972; Electron microscopic characterization of newly isolated Rhizobium lupini bacteriophages. Canadian Journal of Microbiology 18:1271–1274
    [Google Scholar]
  12. Lovett P. S. 1972; PBP1: a flagella specific bacteriophage mediating transduction in Bacillus pumilis. Virology 47:743–752
    [Google Scholar]
  13. Meynell E. W. 1961; A phage, ϕ χ, which attacks motile bacteria. Journal of General Microbiology 25:253–290
    [Google Scholar]
  14. Raimondo L. M., Lundh N. O., Martinez R. J. 1968; Primary adsorption site of phage PBS1: the flagellum of Bacillus subtilis. Journal of Virology 2:256–264
    [Google Scholar]
  15. Schade S. Z., Adler J. 1967; Purification and chemistry of bacteriophage χ. Journal of Virology 1:591–598
    [Google Scholar]
  16. Schade S. Z., Adler J., Ris H. 1967; How bacteriophage χ attacks motile bacteria. Journal of Virology 1:599–609
    [Google Scholar]
  17. Schmitt R., Bamberger I., Acker G., Mayer F. 1974a; Feinstrukturanalyse der komplexen Geiẞeln von Rhizobium lupini H13–3. Archives of Microbiology 100:145–162
    [Google Scholar]
  18. Schmitt R., Lotz W., Mayer F. 1975; Structure of the complex flagella of Rhizobium lupini H13–3. In Proceedings of the First Intersectional Congress of IAMS vol 1 pp 691–695 Edited by Hasegawa T. Tokyo: Science Council of Japan:
    [Google Scholar]
  19. Schmitt R., Raska I., Mayer F. 1974b; Plain and complex flagella of Pseudomonas rhodos: analysis of fine structure and composition. Journal of Bacteriology 117:844–857
    [Google Scholar]
  20. Silverman M. R., Simon M. I. 1972; Flagellar assembly mutants in Escherichia coli. Journal of Bacteriology 112:986–993
    [Google Scholar]
  21. Valentine R. C., Shapiro B. M., Stadtman E. R. 1968; Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry 7:2143–2152
    [Google Scholar]
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