1887

Abstract

Summary

Two split influenza virus vaccines administered intragastrically induced lower titres of haemagglutinin (HA)-specific antibodies in pulmonary secretions than whole virus vaccine or a third split virus vaccine. IgA antibody was the predominant HA-specific Ig class. HA-specific IgA titres decayed substantially within 2 weeks following booster immunization, but persisted for at least another 3.5 months. In contrast, HA-specific IgG was maintained at low titres throughout the 4 month study period. When the total vaccine antigenic mass was administered as one dose or as equally divided doses spread over several days, pulmonary antibody responses were comparable. Mice immunized intragastrically with whole virus vaccine were completely protected against intranasal challenge with a homologous virulent virus of the H3 subtype. Partial protection was obtained when the vaccine used for immunization was a distantly related, antigenically variant strain of the same subtype, but no protection was obtained with a monovalent vaccine of an influenza A subtype different to the challenge virus. These characteristics of the response to intragastric immunization against influenza are consistent with features of a useful vaccine.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-11-2779
1988-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/11/JV0690112779.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-11-2779&mimeType=html&fmt=ahah

References

  1. BERGMAN K.-CH., WALDMAN R. H., TISCHNER H., POHL W.-D. 1986; Antibody in tears, saliva and nasal secretions following oral immunization of humans with inactivated influenza virus vaccine. International Archives of Allergy and Applied Immunology 80:107–109
    [Google Scholar]
  2. CHALLACOMBE S. J. 1983; Salivary antibodies and systemic tolerance in mice after oral immunization with bacterial antigens. Annals of the New York Academy of Sciences 409:177–193
    [Google Scholar]
  3. CHEN K. S., BURLINGTON D. B., QUINNAN G. V. JR 1987; Active synthesis of hemagglutinin-specific IgA by lung cells of mice that were immunized intragastrically with inactivated influenza virus vaccine. Journal of Virology 61:2150–2154
    [Google Scholar]
  4. CLEMENTS M. L., MURPHY B. R. 1986; Development and persistence of local and systemic antibody responses in adults given live attenuated or inactivated influenza A virus vaccine. Journal of Clinical Microbiology 23:66–72
    [Google Scholar]
  5. LAVER W. G., KILBOURNE E. D. 1966; Identification in a recombinant influenza virus of structural proteins derived from both parents. Virology 30:493–501
    [Google Scholar]
  6. LAZZELL V., WALDMAN R. H., ROSE C, KHAKOO R., JACKNOWTTZ A., HOWARD S. 1984; Immunization against influenza in humans using an oral enteric-coated killed virus vaccine. Journal of Biological Standardization 12:315–321
    [Google Scholar]
  7. LIEW F. Y., RUSSELL S. M., APPLEYARD G. A., BRAND C. M., BEALE J. 1984; Cross-protection in mice infected with influenza A virus by the respiratory route is correlated with local IgA antibody rather than serum antibody or cytotoxic T cell reactivity. European Journal of Immunology 14:350–356
    [Google Scholar]
  8. PHELAN M. A., MAYNER R. E., BUCHER D. J., ENNIS F. A. 1980; Purification of influenza virus glycoproteins for the preparation and standardization of immunological potency testing reagents. Journal of Biological Standardization 8:233–242
    [Google Scholar]
  9. SNYDER M. H., CLEMENTS M. L., BETTS R. F., DOLIN R., BUCKLER-WHITE A. J., TIERNEY E. L., MURPHY B. R. 1986; Evaluation of live avian-human reassortant influenza A H3N2 and H1N1 virus vaccines in seronegative adult volunteers. Journal of Clinical Microbiology 23:852–857
    [Google Scholar]
  10. TAYLOR H. P., DIMMOCK N. J. 1985; Mechanism of neutralization of influenza virus by secretory IgA is different from that of monomeric IgA or IgG. Journal of Experimental Medicine 161:198–209
    [Google Scholar]
  11. THEODORSSON-NORHEIM E. 1986; Kruskal-Wallis test: BASIC computer program to perform nonparametric one-way analysis of variance and multiple comparisons on ranks of several independent samples. Computer Methods and Programs in Biomedicine 23:57–62
    [Google Scholar]
  12. WALDMAN R. H., KASEL J. A., FULK R. V., TOGO Y., HORNICK R. B., HEINER G. G., DAWKINS A. T. JR, MAN J. J. 1968; Influenza antibody in human respiratory secretions after subcutaneous or respiratory immunization with inactivated virus. Nature, London 218:594–595
    [Google Scholar]
  13. WILLIAMS M. S., MAYNER R. E., DANIEL N. J., PHELAN M. A., RASTOGI S. C, BOZEMAN F. M., ENNIS F. A. 1980; New developments in the measurement of the hemagglutinin content of influenza virus vaccines by single-radial-immunodiflusion. Journal of Biological Standardization 8:289–296
    [Google Scholar]
  14. WRIGHT P. F., MURPHY B. R., KERVINA M., LAWRENCE E. M., PHELAN M. A., KARZON D. T. 1983; Secretory immunological response after intranasal inactivated influenza A virus vaccinations: evidence for immunoglobulin A memory. Infection and Immunity 40:1092–1095
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-11-2779
Loading
/content/journal/jgv/10.1099/0022-1317-69-11-2779
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error