1887

Abstract

We have constructed six hybrid polioviruses (PVs) modified to express PV type 2 and type 3 antigenic determinants on a PV type 1 (Mahoney) capsid. The hybrids were modified in neutralizing antigenic site (NAg) I and/or NAgII. They were viable, but impaired for growth in comparison to PV1 (Mahoney). Some hybrids modified to express type 2 and type 3 NAgI determinants simultaneously displayed some type 2 but no type 3 antigenicity (in addition to type 1 antigenicity associated with other antigenic sites). Hybrids modified to express a type 2 NAgI determinant and a type 3 NAgII determinant, or vice versa, displayed antigenic characteristics of all three serotypes, although expression of the modified NAgII determinant was weak. We conclude that it is possible to construct a viable hybrid PV simultaneously modified in NAgI and NAgII which expresses antigenic determinants of all three serotypes.

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1992-03-01
2024-04-26
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References

  1. Burke K. L., Dunn G., Ferguson M., Minor P. D., Almond J. W. 1988; Antigen chimeras of poliovirus as potential new vaccines. Nature, London 332:81–82
    [Google Scholar]
  2. Cooper P. D., Agol V. I., Bachrach H. L., Brown F., Ghendon Y., Gibbs A. J., Gillespie J. H., Lonberg-Holm K., Mandel B., Melnick J. L., Mohanty S. B., Povey R. C., Rueckert R. R., Schaffer F. L., Tyrrell D. A. I. 1978; Picomaviridae: second report. Intervirology 10:165–180
    [Google Scholar]
  3. Evans D. J., McKeating J., Meredith J. M., Burke K. L., Katrak K., John A., Ferguson M., Minor P. D., Weiss R. A., Almond J. W. 1989; An engineered poliovirus chimaera elicits broadly reactive HIV-1 neutralizing antibodies. Nature, London 339:385–388
    [Google Scholar]
  4. Golding S. M., Hedger R. S., Talbot P., Watson J. 1976; Radial immunodiffusion and serum neutralization techniques for the assay of antibodies to SVD. Research in Veterinary Science 20:142–147
    [Google Scholar]
  5. Hogle J. M., Filman D. J. 1987; The three dimensional structure of poliovirus: implication for assembly and immune recognition. In Positive Strand RNA Viruses pp 79–92 Edited by Brinton M. A., Rueckert R. R. New York: Alan R. Liss;
    [Google Scholar]
  6. Hogle J. M., Chow M., Filman D. J. 1985; The three dimensional structure of poliovirus at 2-9Ä resolution. Science 229:1358–1365
    [Google Scholar]
  7. Jameson B. A., Wimmer E., Kew O. 1985; Natural variants of the Sabin typel vaccine strain of poliovirus and correlation with a poliovirus neutralization site. Virology 143:337–341
    [Google Scholar]
  8. Jenkins O., Cason J., Burke K. L., Lunney D., Gillen A., Patel D., McCance D. J., Almond J. W. 1990; An antigen chimaera of poliovirus induces antibodies against human papillomavirus type 16. Journal of Virology 64:1201–1206
    [Google Scholar]
  9. Kitson J. D. A., Burke K. L., Pullen L. A., Belsham G. J., Almond J. W. 1991; Chimericpoliovirusesthat includesequences derived from two independent antigenic sites of foot-and-mouth disease virus (FMDV) induce neutralizing antibodies against FMDV in guinea-pigs. Journal of Virology 65:3068–3075
    [Google Scholar]
  10. Martin A., Wychowski C., Couderc T., Crainic R., Hogle J., Girard M. 1988; Engineering a type 2 antigenic site on a type 1 capsid results in a chimeric virus which is neurovirulent for mice. EMBO Journal 7:2839–2847
    [Google Scholar]
  11. Melnick J. L. 1985; Enteroviruses: polioviruses, coxsackie viruses, echoviruses and the newer enteroviruses. In Virology pp 739–794 Edited by Fields B. N. New York: Raven Press;
    [Google Scholar]
  12. Minor P. D., Ferguson M., Schild G. C., Almond J. W., Stanway G. 1987; Molecular basis of antigenicity of poliovirus. In Positive Strand RNA Viruses pp 539–553 Edited by Brinton M. A., Rueckert R. R. New York: Alan R. Liss;
    [Google Scholar]
  13. Minor P. D., Ferguson M., Katrak K., Wood D., John A., Howlett J., Dunn G., Burke K., Almond J. W. 1990; Antigenic chimeras of type 1 and type 3 poliovirus involving antigenic site 1. Journal of General Virology 71:2543–2551
    [Google Scholar]
  14. Murdin A. D., Wimmer E. 1989; Construction of a poliovirus type 1/type 2 antigenic hybrid by manipulation of neutralization antigenic site II. Journal of Virology 63:5251–5257
    [Google Scholar]
  15. Murdin A. D., Kameda A., Murray M. G., Wimmer E. 1991a; Phenotypic characterization of antigenic hybrids of poliovirus. Microbial Pathogenesis 10:39–45
    [Google Scholar]
  16. Murdin A. D., Mirzayan C., Kameda A., Wimmer E. 1991b; The effect of site and mode of expression of a heterologous antigenic determinant on the properties of poliovirus hybrids. Microbial Pathogenesis 10:27–37
    [Google Scholar]
  17. Murray M. G., Wimmer E. 1988; Chemical synthesis of poliovirus peptides and neutralizing antibody responses. Applied Virology Research 1:129–140
    [Google Scholar]
  18. Murray M. G., Bradley J., Yang X.-F., Wimmer E., Moss E. G., Racaniello V. R. 1988a; Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site I. Science 241:213–215
    [Google Scholar]
  19. Murray M. G., Kuhn R. J., Arita M., Kawamura N., Nomoto A., Wimmer E. 1988b; Poliovirus type 1/type 3 antigenic hybrid virus constructed in vitro elicits type 1 and type 3 neutralizing antibodies in rabbits and monkeys. Proceedings of the National Academy of Sciences, U.S.A 85:3203–3207
    [Google Scholar]
  20. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–794
    [Google Scholar]
  21. Palmenberg A. C. 1989; Sequence alignments of picomavirus capsid proteins. In Molecular Aspects of Picornaviral Infection and Detection pp 211–241 Edited by Semler B. L., Ehrenfeld E. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  22. Wiegers K., Uhlig H., Dernick R. 1989; N-AglB of poliovirus type 1: a discontinuous epitope formed by two loops of VP1 comprising residues 96-104 and 141-152. Virology 170:583–586
    [Google Scholar]
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