1887

Abstract

A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of structure. A cDNA for TLP20 was isolated and sequenced. The 3′ non-coding sequence contained a region of high identity with the 5′ end of two other baculovirus genes. The 5′ non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.

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1994-07-01
2024-04-26
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References

  1. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 171:537–555
    [Google Scholar]
  2. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
    [Google Scholar]
  3. Chang C. T., Wu C.-S. C., Yang J. T. 1978; Circular dichroic analysis of protein conformation: inclusion of the beta-turns. Analytical Biochemistry 91:13–31
    [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  5. Hartshorne D. J. 1987; Biochemistry of the contractile process in smooth muscle. In Physiology of the Gastrointestinal Tract pp. 423–482 Johnson L. R. Edited by New York: Raven Press;
    [Google Scholar]
  6. Holden H. M., Ito M., Hartshorne D. J., Rayment I. 1992; X-ray structure determination of telokin, the C-terminal domain of myosin light chain kinase, at 2·8 Å resolution. Journal of Molecular Biology 227:840–851
    [Google Scholar]
  7. Hultmark D., Klemenz R., Gehring W. J. 1986; Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22. Cell 44:429–438
    [Google Scholar]
  8. Ito M., Dabrowska R., Guerriero V. Jr Hartshorne D. J. 1989; Identification in turkey gizzard of an acidic protein related to the C-terminal portion of smooth muscle myosin light chain kinase. Journal of Biological Chemistry 264:13971–13974
    [Google Scholar]
  9. Nagamine T., Sugimori H., Nakamura K., Saga S., Kobayashi M. 1991; Nucleotide sequence of the gene encoding for p40, an occluded virion-specific polypeptide of Bombyx mori nuclear polyhedrosis virus. Journal of Invertebrate Pathology 58:290–293
    [Google Scholar]
  10. Nissen M. S., Friesen P. D. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63:493–503
    [Google Scholar]
  11. Studier W. F., Rosenberg A. H., Dunn J. J., Dubendorff J. W. 1990; Use of T7 polymerase to direct expression of cloned genes. Methods in Enzymology 85:60–89
    [Google Scholar]
  12. Weiher H., Konig M., Gruss P. 1983; Multiple point mutations affecting the simian virus 40 enhancer. Science 219:626–631
    [Google Scholar]
  13. Whitford M., Faulkner P. 1992; Nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 66:4763–4768
    [Google Scholar]
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