1887

Abstract

A deletion mutant of influenza virus haemagglutinin (HA; headless HA) lacking the globular region was expressed in CV-1 cells and detected with a monoclonal antibody, C179, which recognizes a conformational epitope in the middle of the stem region of HA and neutralizes all H1 and H2 subtypes. The cDNA coding for the headless HA was constructed from influenza virus A/Okuda/57 (H2N2), which was also used to select C179. The conformational epitope recognized by C179 was highly stable even after removal of the globular region. The survival rate of mice immunized with the headless HA and challenged with lethal influenza virus A/FM/1/47 (H1N1) was significantly higher than that of the control mice. The headless HA has the potential to induce cross-protection against influenza virus infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-7-1483
1996-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/7/JV0770071483.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-7-1483&mimeType=html&fmt=ahah

References

  1. Carr C. M., Kim P. S. 1993; A spring-loaded mechanism for the conformational change of influenza hemagglutinin. Cell 73:823–832
    [Google Scholar]
  2. Carr C. M., Kim P. S. 1994; Flu virus invasion: halfway there. Science 266:234–236
    [Google Scholar]
  3. Chirgwin J. M., Przybyia A. E., MacDonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  4. Mbawuike I. N., Dillon S. B., Demuth S. G., Jones C. S., Cate T. R., Couch R. B. 1994; Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS1/HA2 influenza virus protein. Vaccine 12:1340–1348
    [Google Scholar]
  5. Mizushima S., Nagata S. 1990; pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Research 18:5322
    [Google Scholar]
  6. Okuno Y., Tanaka K., Baba K., Maeda A., Kunita N., Ueda S. 1990; Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system. Journal of Clinical Microbiology 28:1308–1313
    [Google Scholar]
  7. Okuno Y., Isegawa Y., Sasao F., Ueda S. 1993; A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains. Journal of Virology 67:2552–2558
    [Google Scholar]
  8. Okuno Y., Matsumoto K., Isegawa Y., Ueda S. 1994; Protection against the mouse-adapted A/FM/1/47 strain of influenza A virus in mice by a monoclonal antibody with cross-neutralizing activity among H1 and H2 strains. Journal of Virology 68:517–520
    [Google Scholar]
  9. Takeshita S., Sato M., Toba M., Masahashi W., Hashimoto-Gotoh T. 1987; High-copy-number plasmid vectors for lacZα-comple- mentation and chloramphenicol- or kanamycin resistance selection. Gene 61:63–74
    [Google Scholar]
  10. Tamura S., Ito Y., Asanuma H., Hirabayashi Y., Suzuki Y., Nagamine T., Aizawa C., Kurata T. 1992; Cross-protection against influenza virus infection afforded by trivalent inactivated vaccines inoculated intranasally with cholera toxin B subunit. Journal of Immunology 149:981–988
    [Google Scholar]
  11. Wiley D. C., Skehel J. J. 1987; The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annual Review of Biochemistry 56:365–394
    [Google Scholar]
  12. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature 289:373–378
    [Google Scholar]
  13. Wilson I. A., Skehel J. J., Wiley D. C. 1981; Structure of the haemagglutinin glycoprotein of influenza virus at 3 A resolution. Nature 289:366–373
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-7-1483
Loading
/content/journal/jgv/10.1099/0022-1317-77-7-1483
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error