1887

Abstract

SUMMARY

An endogenous RNA-dependent RNA polymerase has been demonstrated in broccoli necrotic yellows virus (BNYV) particles. The enzyme requires a temperature of 26 °C, a pH value of 7.5, 0.5% NP40 and 4 m-Mg for maximum synthesis of RNA; Mn inhibited the activity. Addition of EDTA to the reaction mixture did not stimulate the activity in the presence of higher amounts of Mg. The transcription products of BNYV hybridized to the virus genome, but not to that of lettuce necrotic yellows virus (LNYV). The transcriptase activity was abolished by centrifugation on a glycerol gradient after treating the virus with NP40 in a high salt solution; combining the pellet and the upper part of the gradient restored the activity. Cross-complementation of BNYV and LNYV transcription could not be obtained by heterologous combination of the pellet and supernatant fractions of the two viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-56-1-59
1981-09-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/56/1/JV0560010059.html?itemId=/content/journal/jgv/10.1099/0022-1317-56-1-59&mimeType=html&fmt=ahah

References

  1. Aaslestad H. G., Clark H. F., Bishop D. H. L., Koprowski H. 1971; Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. Journal of Virology 7:726–735
    [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. 1970; Ribonucleic acid synthesis of vesicular stomatitis virus. II. An RNA polymerase in the virion. Proceedings of the National Academy of Sciences of the United States of America 66:572–576
    [Google Scholar]
  3. Bishop D. H. L., Emerson S. U., Flamand A. 1974; Reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (Indiana serotype), Chandipura, vesicular stomatitis (New Jersey serotype), and Cocal viruses. Journal of Virology 14:139–144
    [Google Scholar]
  4. Breindle M., Holland J. J. 1976; Studies on the in vitro transcription and translation of VSV m-RNA. Virology 73:106–118
    [Google Scholar]
  5. Chang S. H., Hefti E., Obijeski J. F., Bishop D. H. L. 1974; RNA transcription by the virion polymerases of five rhabdoviruses. Journal of Virology 13:652–661
    [Google Scholar]
  6. Emerson S. U., Wagner R. R. 1972; Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions. Journal of Virology 10:297–309
    [Google Scholar]
  7. Flamand A., Delagneau J. F., Bussereau F. 1978; An RNA polymerase activity in purified rabies virions. Journal of General Virology 40:233–238
    [Google Scholar]
  8. Francki R. I. B., Randles J. W. 1972; RNA-dependent RNA polymerase associated with particles of lettuce necrotic yellows virus. Virology 47:270–275
    [Google Scholar]
  9. Kawai A. 1977; Transcriptase activity associated with rabies virion. Journal of Virology 24:826–835
    [Google Scholar]
  10. Lin M. T., Campbell R. N. 1972; Characterization of broccoli necrotic yellows virus. Virology 48:30–40
    [Google Scholar]
  11. McAllister P. E., Wagner R. R. 1977; Virion RNA polymerases of two salmonid rhabdoviruses. Journal of Virology 22:839–843
    [Google Scholar]
  12. Michalski R., Parks N. F., Sokol F., Clark H. F. 1976; Thermal inactivation of rabies and other rhabdoviruses: stabilization by the chelating agent ethylenediaminetetraacetic acid at physiological temperatures. Journal of Virology 20:157–169
    [Google Scholar]
  13. Peters D., Kitajima E. W. 1971; Purification and electron microscopy of sowthistle yellow vein virus. Virology 41:135–150
    [Google Scholar]
  14. Roy P., Clewley J. P. 1978; Spring viremia of carp virus RNA and virion-associated transcriptase activity. Journal of Virology 25:912–916
    [Google Scholar]
  15. Roy P., Clark H. F., Madore H. P., Bishop D. H. L. 1975; RNA polymerase associated with virions of pike fry rhabdovirus. Journal of Virology 15:338–347
    [Google Scholar]
  16. Toriyama S., Peters D. 1980; In vitro synthesis of RNA by dissociated lettuce necrotic yellows virus particles. Journal of General Virology 50:125–134
    [Google Scholar]
  17. Wagner R. R. 1975; Reproduction of rhabdoviruses. In Comprehensive Virology vol 4: pp 1–93 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-56-1-59
Loading
/content/journal/jgv/10.1099/0022-1317-56-1-59
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error