1887

Abstract

SUMMARY

Digestion of the -acetylated-tyrosylated form of brome mosaic virus (BMV) RNA with proteinase K removed the 3′-blocking -acetyl-tyrosine residue. Removal of the blocking group affected neither infectivity nor mRNA activity. The previously reported effect of acetylation of tyrosylated BMV RNA on infectivity appears to result from non-specific acetylation of the RNA.

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1983-09-01
2024-05-18
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References

  1. Agranovsky A. A., Dolja V. V., ATabekov J. G. 1982; Structure of the 3′ extremity of barley stripe mosaic virus RNA: evidence for internal poly(A) and a 3′ terminal tRNA-like structure. Virology 119:51–58
    [Google Scholar]
  2. Ahlquist P., Dasgupta R., Kaesberg P. 1981; Near identity of 3′ RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23:183–189
    [Google Scholar]
  3. Chen J. M., Hall T. C. 1973; Comparison of tyrosyl transfer ribonucleic acid and brome mosaic virus tyrosyl ribonucleic acid as amino acid donors in protein synthesis. Biochemistry 12:4570–4574
    [Google Scholar]
  4. Davies J. W., Kaesberg P. 1974; Translation of virus mRNA: protein synthesis directed by several heterologous viral RNAs in a cell-free extract from wheat germ. Journal of General Virology 25:11–20
    [Google Scholar]
  5. Faulhammer H. G., Cramer F. 1977; Tyrosyl-tRNA synthetase from baker’s yeast: rapid isolation by affinity elution, molecular weight of the enzyme, and determination of essential sulfhydryl groups. European Journal of Biochemistry 75:561–570
    [Google Scholar]
  6. Hall T. C. 1979; Transfer RNA-like structures in viral genomes. In International Review of Cytology vol 60: pp 1–26 Edited by Bourne G. H. Danielli J. F., Jean K. W. New York: Academic Press;
    [Google Scholar]
  7. Joshi S., Haenni A. L., Hubert E., Huez G., Marbaix G. 1978; In vivo aminoacylation and ‘processing’ of turnip yellow mosaic virus RNA in Xenopus laevis oocytes. Nature, London 275:339–341
    [Google Scholar]
  8. Kiberstis P. A., Loesch-Fries L. S., Hall T. C. 1981; Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  9. Kohl R. J., Hall T. C. 1974; Aminoacylation of RNA from several viruses: amino acid specificity and differential activity of plant, yeast, and bacterial synthetases. Journal of General Virology 25:257–261
    [Google Scholar]
  10. Kohl R. J., Hall T. C. 1977; Loss of infectivity of brome mosaic virus RNA after chemical modification of the 3′- or 5′-terminus. Proceedings of the National Academy of Sciences, U. S. A 74:2682–2686
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Loesch-Fries L. S., Hall T. C. 1982; In vivo aminoacylation of brome mosaic and barley stripe mosaic virus RNAs. Nature, London 298:771–773
    [Google Scholar]
  13. Mcleester R. C., Hall T. C. 1977; Simplification of amino acid incorporation and other assays using filter paper techniques. Analytical Biochemistry 79:627–630
    [Google Scholar]
  14. Vidales F. J., Bernabeu C., Ballesta J. P. G. 1979; Peptidyl transfer ribonucleic acid hydrolase activity of proteinase K. Biochemistry 18:4155–1158
    [Google Scholar]
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