1887

Abstract

SUMMARY

A temperature-sensitive mutant ( 1/9) obtained by undiluted passage of fowl plague virus (FPV) at 33 °C carried a strong defect in RNA segment 6 [neuraminidase (NA) gene] and a weak defect in RNA segment 8 [non-structural (NS) protein gene]. Although the viral proteins have normal migration rates, the NS gene migrated during polyacrylamide gel electrophoresis (PAGE) significantly faster than the NS gene of wild-type FPV, even after denaturation by glyoxal. Despite this observation, the NS gene of 1/9 did not carry a deletion as shown by sequence determination. There were only five base replacements which resulted in three changes in amino acids. Three of the base replacements led to a more compact secondary structure of RNA segment 8, which seems to be responsible for the faster migration rate during PAGE and which seems to resist, at least partially, the treatment with glyoxal.

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1985-08-01
2024-04-26
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References

  1. Aminoff D. 1961; Methods of quantitative estimation of N-acetyl neuraminic acid and their application to hydrolysates of sialo mucoids. Biochemical Journal 81:384–392
    [Google Scholar]
  2. Bosch F. X., Orlich M., Klenk H.-D., Rott R. 1979; The structure of the hemagglutinin, a determinant for the pathogenicity of influenza virus. Virology 95:197–207
    [Google Scholar]
  3. Desselberger U., Palese P. 1978; Molecular weights of RNA segments of influenza A and B viruses. Virology 88:394–399
    [Google Scholar]
  4. Floyd R. W., Stone M. P., Joklik W. K. 1974; Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Analytical Biochemistry 59:599–609
    [Google Scholar]
  5. Klenk H.-D., Rott R., Becht H. 1972; On the structure of influenza virus envelope. Virology 47:579–591
    [Google Scholar]
  6. Koennecke I., Boschek C. B., Scholtissek C. 1981; Isolation and properties of a temperature-sensitive mutant (ts 412) of an influenza A virus recombinant with a ts lesion in the gene coding for the nonstructural protein. Virology 110:16–25
    [Google Scholar]
  7. Land H., Grez M., Hauser H., Lindenmaier W., Schätz G. 1981; 5′-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Research 9:2251–2266
    [Google Scholar]
  8. Lobban P. E., Kaiser A. D. 1973; Enzymatic end-to-end joining of DN A molecules. Journal of Molecular Biology 78:453–471
    [Google Scholar]
  9. McMaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U. S. A 74:4835–4838
    [Google Scholar]
  10. Messing J., Vieira J. 1982; A new pair of M13 vectors for selecting either DNA strand of double-digested restriction fragments. Gene 19:269–276
    [Google Scholar]
  11. Peacock S. L., McLver C. M., Monohan I.I. 1981; Transformation of E. coli using homopolymer linked plasmid chimeras. Biochimica et biophysica acta 655:243–250
    [Google Scholar]
  12. Porter A. G., Smith J. C., Emtage J. S. 1980; Nucleotide sequence of influenza virus RNA segment 8 indicates that coding regions for NS 1 and NS2 protein overlap. Proceedings of the National Academy of Sciences, U. S. A 77:5074–5078
    [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U. S. A 74:5463–5467
    [Google Scholar]
  14. Scholtissek C., Müller K. 1983; Temperature-sensitive mutants of fowl plague virus (influenza A) generated by undiluted passages at 33 °C. Journal of General Virology 64:2621–2629
    [Google Scholar]
  15. Scholtissek C., Harms E., Rohde W., Orlich M., Rott R. 1976; Correlation between RNA fragments of fowl plague virus and their corresponding gene functions. Virology 74:332–344
    [Google Scholar]
  16. Scholtissek C., Schwarz R. T., Keil W., Klenk H.-D. 1984; A mutant of fowl plague virus (influenza A) with an altered glycosylation pattern in its hemagglutinin. Virology 136:1–9
    [Google Scholar]
  17. Skehel J. J., Hay A. J. 1978; Influenza virus transcription: review article. Journal of General Virology 39:1–6
    [Google Scholar]
  18. Steuler H., Rohde W., Scholtissek C. 1984; Sequence of the neuraminidase gene of an avian influenza A virus (A/parrot/Ulster/73, H7N1). Virology 135:118–124
    [Google Scholar]
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