1887

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Volume 68, Issue 1

Three Mutants of Herpes Simplex Virus Type 2: One Lacking the Genes US10, US11 and US12 and Two in which R Has Been Extended by 6 kb to 0·91 Map Units with Loss of U Sequences between 0·94 and the U/TR Junction Free

Abstract

Summary

In the process of generating restriction endonuclease site deletion mutants, we have isolated and characterized three mutants of herpes simplex virus type 2 (HSV-2), strain HG52, with large genomic deletions in U and TR. The deleted sequences (7·5 kb) extend from 0·94 map coordinates (m.c.) to 0·99 m.c. and are presumed to contain the HSV-2 gene equivalents of US 10, 11 and 12, one copy of immediate early (IE) gene 3 and one copy of an origin of replication (ORI). One of the mutants (HG52X163X12) has a simple deletion whereas in the two others (HG52X163X14 and HG52X163X21) the deleted sequences have been replaced by inverted duplications of U/IR sequences between 0·83 and 0·91 m.c. such that the molecules have short region inverted repeats extended by 6 kb on either side. All three are viable, stable and grow in tissue culture indicating that the polypeptides coded by the HSV-2 genes equivalent to US10,11 and 12 are non-essential for lytic growth in BHK21/C13 cells. In addition the lack of one copy of the HSV-2 equivalent of IE gene 3 and ORI in HG52X163X12 shows that only one copy of each suffices for viability. However the temperature restriction of the mutants at 38·5 °C suggests that one or more of the polypeptides coded by the deleted sequences may be required in conjunction with another polypeptide(s) for viral growth or stability at the higher temperature.

  • Received:
  • Accepted:
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© The Journal of General Virology 1987
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1987-01-01
2024-04-28
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Three Mutants of Herpes Simplex Virus Type 2: One Lacking the Genes US10, US11 and US12 and Two in which RS Has Been Extended by 6 kb to 0·91 Map Units with Loss of US Sequences between 0·94 and the US/TRS Junction
J. Gen. Virol. 68, 1 (1987); https://doi.org/10.1099/0022-1317-68-1-1
/content/journal/jgv/10.1099/0022-1317-68-1-1
/content/journal/jgv/10.1099/0022-1317-68-1-1
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