1887

Abstract

Summary

The genomic RNA (6·2 kb) and a 0·9 kb RNA were detected by Northern hybridization in leaves infected with white clover mosaic virus (WC1MV). The 0·9 kb RNA was efficiently encapsidated by one, but not by eight other, isolates of WC1MV. Both RNA species were shown to possess 3′-terminal tracts of poly (A) 200 to 300 nucleotides long by end-labelling with [P]pCp and digestion with ribonucleases A, Phy M, T, and U. The 0·9 kb RNA was mapped to the 3′-terminal region of the genomic RNA using cDNA probes. In rabbit reticulocyte lysates, the 6·2 kb RNA directed predominantly the synthesis of a protein of mol. wt. 160000 (160K) and several additional proteins. The 0·9 kb RN A directed synthesis of a 25K protein. This protein was shown to be the virus coat protein by its co-migration in gels with the WC1MV coat protein and by its specific immunoprecipitation with antiserum to virus particles. Both RNA species were found in specific association with polysomes indicating that they function as messenger RNAs in infected cells.

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1987-01-01
2024-05-10
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References

  1. Abouhaidar M. O. 1983; The structure of the 5′and 3′ends of clover yellow mosaic virus RNA. Canadian Journal of Microbiology 29:151–156
    [Google Scholar]
  2. Abouhaidar M., Bancroft J. B. 1978; The structure of the 5′-terminus of papaya mosaic virus RNA. Journal of General Virology 39:559–563
    [Google Scholar]
  3. Adams S. E., Jones R. A. C., Counts R. H. A. 1985; Infection of protoplasts derived from potato shoot cultures with potato virus X. Journal of General Virology 66:1341–1346
    [Google Scholar]
  4. Barker R. F., Jarvis N. P., Thompson D. v., Loesch-Fries L. S., Hall T. C. 1983; Complete nucleotide sequence of alfalfa mosaic virus RNA3. Nucleic Acids Research 11:2881–2891
    [Google Scholar]
  5. Bendena W. G., Abouhaidar M., Mackie G. A. 1985; Synthesis in vitro of the coat protein of papaya mosaic virus. Virology 140:257–268
    [Google Scholar]
  6. Brandes J., Bercks R. 1965; Gross morphology and serology as a basis for classification of elongated plant viruses. Advances in Virus Research 11:1–24
    [Google Scholar]
  7. Brederode F. TH., Koper-Zwarthoff E. C., Bol J. F. 1980; Complete nucleotide sequence of alfalfa mosaic virus RNA 4. Nucleic Acids Research 8:2213–2223
    [Google Scholar]
  8. Cann A. J., Stanway G., Hauptmann R., Minor P. D., Schild G. C., Clarke L. D., Mountford R. C., Almond J. W. 1983; Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins. Nucleic Acids Research 11:1267–1281
    [Google Scholar]
  9. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  10. Cornelissen B. J. C., Brederode F. TH., Moormann R. J. M., Bol J. F. 1983a; Complete nucleotide sequence of alfalfa mosaic virus RNA 1. Nucleic Acids Research 11:1253–1265
    [Google Scholar]
  11. cornelissen B. J. C., Brederode F. TH., Veeneman G. H., Van Boom J. H., Bol J. F. 1983b; Complete nucleotide sequence of alfalfa mosaic virus RNA 2. Nucleic Acids Research 11:3019–3025
    [Google Scholar]
  12. England T. E., Uhlenbeck O. C. 1978; 3′ Terminal labelling of RNA with T4 RNA ligase. Nature; London: 275560–561
    [Google Scholar]
  13. Forster R. L. S., Morris-Krsinich B. A. M. 1985; Synthesis and processing of the translation products of tobacco ringspot virus in rabbit reticulocyte lysates. Virology 144:516–519
    [Google Scholar]
  14. Francki R. I. B., Mclean G. D. 1968; Purification of potato virus X and preparation of infectious ribonucleic acid by degradation with lithium chloride. Australian Journal of Biological Sciences 21:1311–1318
    [Google Scholar]
  15. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  16. Guilford P. J., Forster R. L. S. 1986; Detection of polyadenylated subgenomic RNAs in leaves infected with the potexvirus daphne virus X. Journal of General Virology 67:83–90
    [Google Scholar]
  17. Jackson A. O., Larkins B. A. 1976; Influence of ionic strength, pH, and chelation of divalent met als on isolation of polyribosomes from tobacco leaves. Plant Physiology 57:5–10
    [Google Scholar]
  18. Koenig R. 1971; Nucleic acids in the potato virus X group and in some other plant viruses: comparison of the molecular weights by electrophoresis in acrylamide-agarose composite gels. Journal of General Virology 10:111–114
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Morozov S., Yu, Zakhariev V. M., Chernov B. K., Prasolov V. S., Kozlov, Yu V., Atabekov J. G., Skyabin K. G. 1983; Analysis of primary structure and localization of the coat protein gene in genomic RN A of potato X virus. Doklady Academii nauk SSSR 271:211–215
    [Google Scholar]
  21. Morris-Krsinich B. A. M., Forster R. L. S., Mossop D. W. 1983; Translation of red clover necrotic mosaic virus RNA in rabbit reticulocyte lysate: identification of the virus coat protein cistron on the larger RNA strand of the bipartite genome. Virology 124:349–356
    [Google Scholar]
  22. Murant A. F., Taylor M, Duncan G. H., Raschké J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  23. Neeleman L., Lindhout P., Van Vloten-Doting L. 1985; Artifacts are responsible for the translational activity of polyribosome preparations isolated from alfalfa mosaic virus-infected tobacco leaves. Virology 147:422–430
    [Google Scholar]
  24. Palukaitis P., García-Arenal F., Sulzinski M. A., Zaitlin M. 1983; Replication of tobacco mosaic viruS. VII. Further characterization of single- and double-stranded virus-related RNAs from TMV-infected plants. Virology 131:533–545
    [Google Scholar]
  25. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  26. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  27. Short M. N., Davies J. W. 1983; Narcissus mosaic virus: a potexvirus with an encapsidated subgenomic messenger RNA for coat protein. Bioscience Reports 3:837–846
    [Google Scholar]
  28. Sonenbero N., Shatkin A. J., Ricciardi R. P., Rubin M., Goodman R. M. 1978; Analysis of terminal structures of RNA from potato virus X. Nucleic Acids Research 5:2501–2512
    [Google Scholar]
  29. Szybiak U., Legocki A. B. 1981; Translation in vitro of ribonucleic acids from potato virus X, potato virus M and white clover mosaic virus. Acta biochimica polonica 28:99–104
    [Google Scholar]
  30. Vance V. B., Beachy R. N. 1984; Detection of genomic-length soybean mosaic virus RNA on polyribosomes of infected soybean leaves. Virology 138:26–36
    [Google Scholar]
  31. Vournakis J. N., Efstratiadis A., Kafatos F. C. 1975; Electrophoretic patterns of deadenylated chorion and globin mRNAS. Proceedings of the National Academy of Sciences, U.S.A 72:2959–2963
    [Google Scholar]
  32. Winter G., Brownlee G. G. 1978; 3′ End labelling of RNA with 32P suitable for rapid gel sequencing. Nucleic Acids Research 5:3129–3139
    [Google Scholar]
  33. Wodnar-Filipowicz A., Skrzeczkowski L. J., Filipowicz W. 1980; Translation of potato virus X RNA into high molecular weight proteins. FEBS tetters 109:151–155
    [Google Scholar]
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