1887

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Volume 68, Issue 6

Nuclear DNA-binding Proteins Determined by the Epstein-Barr Virus-related Simian Lymphotropic Herpesviruses and Free

Abstract

Summary

Nuclear DNA-binding proteins were extracted from lymphoblastoid cell lines transformed with Epstein-Barr virus (EBV) or with the related lymphotropic herpesviruses of gorilla (), chimpanzee (), baboon () or orang-utan (). They were immunoblotted with the sera of all four simian species in comparison with EBV antibody-positive human sera. Eight nuclear proteins were identified, and were designated GONA-1 and GONA-2 for -determined nuclear antigens, PANA-1A, PANA-1B and PANA-2 for , PONA-2 for and HUPNA-1 and HUPNA-2 for .papio-determined nuclear antigens. One of two tested HUPNA-2-positive baboon sera and one PONA-2-positive orang-utan serum also reacted with EBNA-2 in EBV-transformed cells. A human serum that contained antibodies to all five EBNA proteins cross-reacted only with PANA-2 and PONA-2. Monospecific anti-peptide antibodies against EBNA-2, type A, also reacted with PONA-2, but not with the other simian nuclear antigens. The data provide evidence that EBV-like simian lymphotropic herpesviruses induce EBNA-like nuclear antigens and that EBNA-2 and some simian EBNA-related proteins contain an epitope that has been conserved during the evolution of the EBV family of viruses.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): herpesvirus and simian/nuclear proteins/EBV
© The Journal of General Virology 1987
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1987-06-01
2024-05-02
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Nuclear DNA-binding Proteins Determined by the Epstein-Barr Virus-related Simian Lymphotropic Herpesviruses H. gorilla, H. pan, H. pongo and H. papio
J. Gen. Virol. 68, 1587 (1987); https://doi.org/10.1099/0022-1317-68-6-1587
/content/journal/jgv/10.1099/0022-1317-68-6-1587
/content/journal/jgv/10.1099/0022-1317-68-6-1587
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