1887

Abstract

Summary

DNA complementary to the tenth largest (S10) of the 12 genome segments of rice dwarf virus (RDV) was cloned and its sequence was determined. This genome segment is 1319 nucleotides in length and has a single long open reading frame extending for 1059 nucleotides from the first AUG triplet (residues 27 to 29). The predicted translation product comprises 352 amino acids and has a mol. wt. of 39094. RDV transcripts synthesized have the same polarity as the plus strand of the genome. Terminal sequences were (+) 5′ GGUA---UGAU 3′ and (-) 3′ CCAU---ACUA 5′ which are similar to those of wound tumour virus RNA.

Keyword(s): dsRNA , nucleotide sequence and RDV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-1-227
1988-01-01
2024-05-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/1/JV0690010227.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-1-227&mimeType=html&fmt=ahah

References

  1. ASAMIZU T., SUMMERS D., MOTIKA M. B., ANZOLA J. V., NUSS D. L. 1985; Molecular cloning and characterization of the genome of wound tumor virus: a tumor-inducing plant reovirus. Virology 144:398–409
    [Google Scholar]
  2. BOCCARDO G., MILNE R. G. 1984; Plant reovirus group. Commonwealth Mycological Institute / Association of Applied Biologists Descriptions of Plant Viruses294
    [Google Scholar]
  3. BODKIN D. K., KNUDSON D. L. 1985; Assessment of sequence relatedness of double-stranded RNA genes by RNA-RNA blot hybridization. Journal of Virological Methods 10:42–52
    [Google Scholar]
  4. CASHDOLLAR L. W., ESPARZA J., HUDSON G. R., CHMELO R., LEE P. W. K., JOKLIK W. K. 1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U.S.A 79:7644–7648
    [Google Scholar]
  5. GOMATOS P. J., STAMATOS N. M., SARKAR N. H. 1980; Small reovirus-specific particle with polycytidylate-dependent RNA polymerase activity. Journal of Virology 36:556–565
    [Google Scholar]
  6. KANEHISA M. I. 1982; Los Alamos sequence analysis package for nucleic acids and proteins. Nucleic Acids Research 10:183–196
    [Google Scholar]
  7. KIMURA I., MINOBE Y., OMURA T. 1987; Changes in a nucleic acid and a protein component of rice dwarf virus particles associated with an increase in symptom severity. Journal of General Virology 68:3211–3215
    [Google Scholar]
  8. KODAMA T., SUZUKI N. 1973; RNA polymerase activity in purified rice dwarf virus. Annals of the Phytopathological Society of Japan 39:251–258
    [Google Scholar]
  9. KOZAK M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9:5233–5252
    [Google Scholar]
  10. LIPMAN D. J., PEARSON W. R. 1985; Rapid and sensitive protein similarity searches. Sciences 227:1435–1441
    [Google Scholar]
  11. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. MESSING J., CREA R., SEEBURG P. H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  13. MINOBE Y., MORI H., OMURA T. 1986; Cloning of the cDNA of double-stranded RNA genomes. Biochemistry 58:1028 Abstract, in Japanese
    [Google Scholar]
  14. MIURA K. 1981; The cap structure in eukaryotic messenger RNA as a mark of a strand carrying protein information. Advances in Biophysics 14:205–238
    [Google Scholar]
  15. MIZUSAWA S., NISHIMURA S., SEELA F. 1986; Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Research 14:1319–1324
    [Google Scholar]
  16. NAKATA M., FUKUNAGA K., SUZUKI N. 1978; Polypeptide components of rice dwarf virus. Annals of the Phytopathological Society of Japan 44:288–296
    [Google Scholar]
  17. OMURA T., INOUE H. 1985; Rice gall dwarf virus. Commonwealth Mycological Institute / Association of Applied Biologists Descriptions of Plant Viruses296
    [Google Scholar]
  18. RHODES D. P., REDDY D. V. R., MACLEOD R., BLACK L. M., BANERJEE A. K. 1977; In vitro synthesis of RNA containing 5′-terminal structure 7mG(5′)Apm by purified wound tumor virus. Virology 76:554–559
    [Google Scholar]
  19. RICHARDSON M. A., FURUICHI Y. 1983; Nucleotide sequence of reovirus genome segment S3, encoding nonstructural protein sigma NS. Nucleic Acids Research 11:6399–6408
    [Google Scholar]
  20. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  21. SHATKIN A. J., KOZAK M. 1982 Biochemical aspects of reovirus transcription and translation. The Reoviridae79–106 Edited by Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  22. SHIMOTOHNO K., MIURA K. 1975; Modified structure in messenger RNA of rice dwarf virus. Report. National Institute of Genetics 26:13
    [Google Scholar]
  23. SMITH R. E., FURUICHI Y. 1980; Gene mapping of cytoplasmic polyhedrosis virus of silkworm by the full-length mRNA prepared under optimized conditions of transcription in vitro. Virology 103:279–290
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-1-227
Loading
/content/journal/jgv/10.1099/0022-1317-69-1-227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error