1887

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Volume 69, Issue 11

Mechanism of Recognition of Herpes Simplex Virus Type 1-infected Cells by Natural Killer Cells Free

Abstract

Summary

Human fibroblast FS-4 cells when infected with herpes simplex virus type 1 (HSV-1) become susceptible to lysis by purified populations of T3 human natural killer (NK) lymphocytes. Blocking of HSV-1 protein synthesis or -linked glycosylation with pactamycin or tunicamycin, respectively, prevented HSV-1-infected cells from being lysed, suggesting that HSV-1 glycoprotein synthesis is required for recognition by NK cells. However, pactamycin- and tunicamycin-treated cells expressed on their membranes a detectable amount (20 to 40% of the untreated control) of HSV-1 glycoproteins gB, gC and gD, left by the virus during its internalization. Phosphonoformic acid (PFA) blocked HSV-1 DNA replication and inhibited the synthesis and surface expression of newly made gC, gD and gB by 90, 80 and 60% respectively. Despite this reduction, PFA treatment had no effect on NK susceptibility. The target structure recognized seems to be different from those expressed on tumour target cells since there was no competition for the lysis of HSV-1-infected FS-4 by K-562 or HeLa tumour target cells. However, a monoclonal antibody specific for the human transferrin receptor which inhibited NK recognition of tumour cells also blocked NK cytotoxicity of HSV-1-infected cells. In summary our results indicate that although viral glycoprotein synthesis is required, gB, gC and/or gD alone are not the targets for NK recognition of HSV-1-infected cells. In addition, they suggest the involvement of the host cell transferrin receptor in the NK killing process.

  • Received:
  • Accepted:
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Keyword(s): HSV-1 , immune response and killer T cells
© Society for General Microbiology 1988
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1988-11-01
2024-04-30
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References

  1. ALARCÓN B., FRESNO M. 1985; Specific effects of anti-transferrin antibodies on natural killer cells directed against tumor cells. Evidence for the transferrin receptor being one of the target structures recognized by NK cells. Journal of Immunology 134:1286–1291
     [Google Scholar]
  2. BIRON C. A., WELSH R. M. 1982; Activation and role of natural killer cells in virus infections. Medical Microbiology and Immunology 170:155–172
     [Google Scholar]
  3. BIRON C. A., TURGISS I. R., WELSH R. M. 1983; Increase in NK cell number and turnover rate during acute viral infection. Journal of Immunology 131:1539–1545
     [Google Scholar]
  4. BISHOP O. A., GLORIOSO J. C., SCHWARTZ S. A. 1983a; Relationship between expression of herpes simplex virus glycoproteins and susceptibility of target cells to human natural killer activity. Journal of Expérimental Medicine 157:1544–1561
     [Google Scholar]
  5. BISHOP G. A., GLORIOSO J. C., SCHWARTZ S. A. 1983b; Role of interferon in human natural killer activity against target cells infected with HSV-1. Journal of Immunology 131:1849–1853
     [Google Scholar]
  6. BISHOP G. A., MARLIN S. D., SCHWARTZ S. A., GLORIOSO J. C. 1984; Human natural killer cell recognition of herpes simplex virus type 1 glycoproteins: specificity analysis with the use of monoclonal antibodies and antigenic variants. Journal of Immunology 133:2206–2214
     [Google Scholar]
  7. BISHOP G. A., KUMEL G., SCHWARTZ S. A., GLORIOSO J. C. 1986; Specificity of human natural killer cells in limiting dilution cultures for determinants of Herpes Simplex virus type 1 glycoproteins. Journal of Virology 57:294–300
     [Google Scholar]
  8. BORYSIEWICZ L. K., RODGERS B., MERRIS S., GRAHAM S., SISSONS J. G. P. 1985; Lysis of human cytomegalovirus infected fibroblasts by natural killer cells: demonstration of an interferon-independent component requiring expression of early viral proteins and characterization of the effector cells. Journal of Immunology 134:2695–2701
     [Google Scholar]
  9. BORYSIEWICZ L. K., GRAHAM S., SISSONS J. G. P. 1986; Human natural killer cell lysis of virus-infected cells. Relationship to expression of the transferrin receptor. European Journal of Immunology 16:405–411
     [Google Scholar]
  10. BUKOWSKI J. F., WELSH R. M. 1986; The role of natural killer cells and interferon in resistance to acute infection of mice with herpes simplex virus type 1. Journal of Immunology 136:3481–3485
     [Google Scholar]
  11. CASALI P., SISSONS J. G. P., BUCHMEIER M. J., OLDSTONE M. B. A. 1981; In vitro generation of human cytotoxic lymphocytes by virus. Journal of Experimental Medicine 154:840–855
     [Google Scholar]
  12. CHING C., LÓPEZ C. 1979; Natural killing of herpes simplex virus type 1-infected target cells: normal human responses and influence of antiviral antibody. Infection and Immunity 26:49–56
     [Google Scholar]
  13. COLMENARES C., LÓPEZ C. 1986; Enhanced lysis of herpes simplex virus type 1-infected mouse cell lines by NC or NK effectors. Journal of Immunology 136:3473–3480
     [Google Scholar]
  14. FITZGERALD P. A., VON WUSSOW P., LÓPEZ C. 1982; Role of interferon in natural killer or HSV-1-infected fibroblasts. Journal of Immunology 129:819–823
     [Google Scholar]
  15. FITZGERALD P. A., EVANS R, KIRPATRICK D., LÓPEZ C. 1983; Heterogeneity of human NK cells: comparison of effectors that lyse HSV-1-infected fibroblasts and K 562 erythroleukemia targets. Journal of Immunology 130:1663–1667
     [Google Scholar]
  16. FRAKER P. J., SPECK J. C. 1978; Protein and cell membrane iodinations with a sparingly soluble chloramide, 1,3,4,6-tetrachloro-3α6α-diphenyl glycouril. Biochemical and Biophysical Research Communications 80:849–856
     [Google Scholar]
  17. GLORIOSO J., SZCZESIUL M. S., MARLIN S. D., LEVINE M. 1983; Inhibition of glycosylation of herpes simplex virus glycoproteins: identification of antigenic and immunogenic partially glycosylated glycopeptides on the cell surface membrane. Virology 126:1–18
     [Google Scholar]
  18. HABU S., AKAMATSU K., TAMAOKI N., OKUMURA K. 1984; In vivo significance of NK cell on resistance against virus (HSV-1) infections in mice. Journal of Immunology 133:2743–2747
     [Google Scholar]
  19. KIESSLING R., WIGZELL H. 1981; Surveillance of primitive cells by natural killer cells. Current Topics in Microbiology and Immunology 92:107–123
     [Google Scholar]
  20. LANIER L. L., PHILLIPS J. H. 1986; Evidence for three types of human cytotoxic lymphocyte. Immunology Today 7:132–134
     [Google Scholar]
  21. LIEBSON P. J., LAZLO M. H., HAYWARD A. R. 1986; Inhibition of herpes simplex type 1 replication in fibroblast cultures by human blood mononuclear cells. Journal of Virology 57:976–982
     [Google Scholar]
  22. LÓPEZ C, RYSHKE R., BENNETT M. 1980; Marrow-dependent cells depleted by 85Sr mediate genetic resistance to herpes simplex virus infection. Infection and Immunity 28:1028–1035
     [Google Scholar]
  23. MUNOZ A., CARRASCO L. 1984; Formation of non-infective herpesvirus particles in cultured cells treated with human interferon. Journal of General Virology 65:1069–1078
     [Google Scholar]
  24. MUNOZ A., CARRASCO L., FRESNO M. 1983; Enhancement of susceptibility of HSV-1 infected cells to natural killer lysis by interferon. Journal of Immunology 131:783–787
     [Google Scholar]
  25. NABEL G., FRESNO M., CHESSMAN A., CANTOR H. 1981; Use of cloned lymphocytes to analyze cellular differentiation. Cell 23:19–28
     [Google Scholar]
  26. NORRILD B., PEDERSEN B. 1982; Effect of tunicamycin on the synthesis of herpes simplex virus type 1 glycoproteins and their expression on the cell surface. Journal of Virology 43:395–402
     [Google Scholar]
  27. ORTALDO J. R., HERBERMAN R. B. 1984; Heterogeneity of natural killer cells. Annual Review of Immunology 2:359–394
     [Google Scholar]
  28. PEAKE M. L., NYSTROM P., PIZER L. 1982; A study of herpes simplex virus glycoprotein synthesis and insertion into plasma membranes. Journal of Virology 42:678–690
     [Google Scholar]
  29. PERRIN D., STÜNZI H. 1984 Antiviral actions of metal ions and metal-chelating agents. Viral Chemotherapy 1255–302 Edited by Shugar D. Oxford & New York: Pergamon Press;
     [Google Scholar]
  30. RAGER-ZISMAN B., QUAN P. C, ROSNER M., MOLLER J. R., BLOOM B. R. 1987; Role of NK cells in protection of mice against herpes simplex virus-1-infection. Journal of Immunology 138:884–888
     [Google Scholar]
  31. SCHWARZ R. T., DATEMA R. 1980; Inhibitors of protein glycosylation. Trends in Biochemical Sciences 5:65–67
     [Google Scholar]
  32. SPEAR P. G. 1985 Glycoproteins specified by herpes simplex virus. The Herpesviruses 3315–347 Edited by Roizman B. New York & London: Plenum Press;
     [Google Scholar]
  33. VODINELICH L., SUTHERLAND R., SCHNEIDER C, NEWMAN R., GREAVES M. 1983; Receptor for transferrin may be a “target” structure for natural killer cells. Proceedings of the National Academy of Sciences, U.S.A 80:835–839
     [Google Scholar]
  34. WAGNER E. K. 1985 Individual HSV transcripts: characterization of specific genes. The Herpesviruses 345–104 Edited by Roizman B. New York & London: Plenum Press;
     [Google Scholar]
  35. WELSH R. M. 1978; Mouse natural killer cells: induction, specificity and functions. Journal of Immunology 121:1631–1636
     [Google Scholar]
  36. WELSH R. M. 1981; Natural cell-mediated immunity during viral infections. Current Topics in Microbiology 192:83–106
     [Google Scholar]
  37. YAKUSAWA M., ZARLING J. M. 1983; Autologous herpes simplex virus-infected cells are lysed by human natural killer cells. Journal of Immunology 131:2011–2016
     [Google Scholar]
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Mechanism of Recognition of Herpes Simplex Virus Type 1-infected Cells by Natural Killer Cells
J. Gen. Virol. 69, 2859 (1988); https://doi.org/10.1099/0022-1317-69-11-2859
/content/journal/jgv/10.1099/0022-1317-69-11-2859
/content/journal/jgv/10.1099/0022-1317-69-11-2859
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