1887

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Volume 70, Issue 6

Target and Effector Cell Fusion Accounts for B Lymphocyte-mediated Lysis of Mouse Hepatitis Virus-infected Cells Free

Abstract

SUMMARY

In confirmation of previous reports, we observed lysis of mouse hepatitis virus (MHV)-infected target cells in the presence of spleen and lymph node cells from non-immunized mice possessing a B cell surface phenotype (IgM, IgG, J11d, Ia, Fc, Thy1, MAC-1 and asialo-GM1). Lysis was inhibited by MHV-specific antisera. The presence of immunoglobulin at the surface of B cells is not required for cytolysis since MHV-infected target cells are lysed in the presence of the B cell hybridoma Sp2/0, which fails to synthesize immunoglobulin. Using Cr-labelled Sp2/0 cells, both target and effector cells were shown to undergo cytolysis. Direct observation of target and effector cells co-incubated after labelling with different fluorescent dyes demonstrated that lysis correlates with the fusion of B cells and MHV-infected cells. These findings are consistent with the idea that the E2 protein of MHV, which is expressed on the infected cell surface and has receptor and membrane-fusion activities at neutral pH, selectively mediates fusion with cells of B lymphocyte lineage. This may represent a general mechanism by which enveloped viruses with fusion proteins that function at neutral pH can interfere with the function of subsets of immune cells.

  • Received:
  • Accepted:
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Keyword(s): fusion-mediated lysis , lymphocytes and MHV
© Society for General Microbiology 1989
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1989-06-01
2024-04-26
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Target and Effector Cell Fusion Accounts for B Lymphocyte-mediated Lysis of Mouse Hepatitis Virus-infected Cells
J. Gen. Virol. 70, 1465 (1989); https://doi.org/10.1099/0022-1317-70-6-1465
/content/journal/jgv/10.1099/0022-1317-70-6-1465
/content/journal/jgv/10.1099/0022-1317-70-6-1465
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