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Volume 71, Issue 12

Equine Herpesvirus Type 1 Unique Short Fragment Encodes Glycoproteins with Homology to Herpes Simplex Virus Type 1 gD, gI and gE Free

Abstract

The nucleotide sequence of a 6·4 kbp portion of the 10·6 kbp HI fragment D contained in the unique short region of the equine herpesvirus type 1 (EHV-1) genome has been determined. Analysis of this sequence revealed five open reading frames (ORFs), four complete and one incomplete, which were encoded by the same sense strand. Comparison of the EHV-1 DNA sequence with that encoding glycoproteins of other alphaherpesviruses has revealed no significant homologies. Comparison at the amino acid level, however, has demonstrated regions of significant sequence similarity between the three complete EHV-1 ORFs 2, 3 and 4, and the herpes simplex virus type 1 (HSV-1) glycoprotein gD encoded by the US6 gene, the HSV-1 glycoprotein gI encoded by the US7 gene and the HSV-1 glycoprotein gE encoded by the US8 gene, respectively. The interrupted ORF 5 was found to display partial homology with the HSV-1 US9- encoded protein, but no homology was found between the protein encoded by ORF 1 and other proteins. The three collinear EHV-1 ORFs encoding putative glycoproteins with homology to the HSV-1 glycoproteins were therefore designated EHV-1 gD, gI and gE, respectively. Moreover, further similarities were found between EHV-1 gD and pseudorabies virus (PRV) gp50, between EHV-1 gI and PRV gp63 and varicella- zoster virus (VZV) gpIV, and between EHV-1 gE and PRV gI and VZV gpI. It is concluded that EHV-1, PRV, HSV-1 and VZV encode homologous glycoprotein genes in the small unique components of their genomes and that the genetic organization of these regions is conserved.

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© Society for General Microbiology 1990
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1990-12-01
2024-05-04
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References

  1. Allen G. P., Bryans J. T. 1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus 1 infections. Progress in Veterinary Microbiology and Immunology 2:78–144
     [Google Scholar]
  2. Allen G. P., Yeargan M. R. 1987; Use of lambda gtll and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus 1. Journal of Virology 61:2454–2461
     [Google Scholar]
  3. Allen G. P., Coogle L. D. 1988; Characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gpl3) with homology to herpes simplex virus glycoprotein C. Journal of Virology 62:2850–2858
     [Google Scholar]
  4. Ben Porat T., DeMarchi J., Lomniczi B., Kaplan A. 1986; Role of glycoproteins of pseudorabies virus in eliciting neutralizing antibodies. Virology 154:325–334
     [Google Scholar]
  5. Bryans J. T. 1978; Immunization of pregnant mares with an inactivated equine herpesvirus 1 vaccine. In Equine Infectious Diseases 4 pp. 83–92 Bryans J., Gerber H. Edited by Princeton: Veterinary Publications;
     [Google Scholar]
  6. Bryans J. T., Allen G. P. 1986; Control of abortigenic herpesviral infections. In Current Therapy in Theriogeneology 2 pp. 711–714 D. Morrow. Edited by Philadelphia: W. B. Saunders;
     [Google Scholar]
  7. Cantin E. M., Eberle R., Baldick J. L., Moss B., Willey D. E., Notkins A. L., Openshaw H. 1987; Expression of herpes simplex virus 1 glycoprotein B by a recombinant vaccinia virus and protection of mice against lethal herpes simplex virus infection. Proceedings of the National Academy of Sciences, U.S.A 84:5908–5912
     [Google Scholar]
  8. Cullinane A. A., Rixon F. J., Davison A. J. 1988; Characterization of the genome of equine herpesvirus 1 subtype 2. Journal of General Virology 69:1575–1590
     [Google Scholar]
  9. Davison A. J. 1983; DNA sequence of the Us component of the varicella-zoster virus genome. EMBO Journal 2:2203–2209
     [Google Scholar]
  10. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
     [Google Scholar]
  11. Glorioso J., Schröder C. H., Kumel G., Szcyesiul M., Levine M. 1984; Immunogenicity of herpes simplex virus glycoproteins gC and gB and their role in protective immunity. Journal of Virology 50:805–812
     [Google Scholar]
  12. Guo P., Goebel S., Davis S., Perkus M. E., Languet B., Desmettre P., Allen G., Paoletti E. 1989; Expression in recombinant vaccinia virus of the equine herpesvirus 1 gene encoding glycoprotein gpl3 and protection of immunized animals. Journal of Virology 63:4189–4198
     [Google Scholar]
  13. Guo P., Goebel S., Perkus M. E., Taylor J., Norton E., Allen G., Languet B., Desmettre P., Paoletti E. 1990; Coexpression by vaccinia virus recombinants of equine herpesvirus-1 glycoproteins gpl3 and gpl4. Journal of Virology 64:2399–2406
     [Google Scholar]
  14. Hattori M., Sakaki Y. 1986; Dideoxy sequencing method using denatured templates. Analytical Biochemistry 152:1015–1026
     [Google Scholar]
  15. Henry B. E., Robinson R. A., Dauenhauer S. A., Atherton S. S., Hayward G. S., O’Callaghan D. J. 1981; Structure of the genome of equine herpesvirus type 1. Virology 115:97–114
     [Google Scholar]
  16. Kost T. A., Jones E. V., Smith K. M., Reed A. P., Brown A. L., Miller T. J. 1989; Biological evaluation of glycoproteins mapping to two distinct mRNAs within the BamHI fragment 7 of pseudorabies virus: expression of the coding regions by vaccinia virus. Virology 171:365–376
     [Google Scholar]
  17. Kozak M. 1983; Comparison of the initiation of protein synthesis in procaryotes, eucaryotes and organelles. Microbiological Reviews 47:1–45
     [Google Scholar]
  18. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
     [Google Scholar]
  19. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
     [Google Scholar]
  20. Longnecker R., Chatterjee S., Whitley R. J., Roizman B. 1987; Identification of a herpes simplex virus 1 glycoprotein gene within a gene cluster dispensable for growth in cell culture. Proceedings of the National Academy of Sciences, U.S.A 84:4303–4307
     [Google Scholar]
  21. McGeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
     [Google Scholar]
  22. O’Callaghan D. J., Gentry G. A., Randall C. C. 1983; The equine herpesviruses. In The Herpesviruses 2 pp. 215–318 Roizman B. Edited by New York: Plenum Press;
     [Google Scholar]
  23. Petrovskis E. A., Timmins J. G., Armentrout M. A., Marchioli C. C., Yancey R. J.JR Post L. E. 1986a; DNA sequence of the gene for pseudorabies virus gp50, a glycoprotein without N-linked glycosylation. Journal of Virology 59:216–223
     [Google Scholar]
  24. Petrovskis E. A., Timmins J. G., Post L. E. 1986b; Use of lambda gtl 1 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins. Journal of Virology 60:185–193
     [Google Scholar]
  25. Proudfoot N. J., Brownlee G. G. 1976; 3′-non-coding region sequences in eukaryotic messenger RNA. Nature; London: 263211–214
     [Google Scholar]
  26. Purdy C. W., Ford S. J., Porter R. C. 1978; Equine rhinopneumonitis vaccine: immunogenicity and safety in adult horses, including pregnant mares. American Journal of Veterinary Research 39:377–383
     [Google Scholar]
  27. Riggio M. P., Cullinane A. A., Onions D. E. 1989; Identification and nucleotide sequence of the glycoprotein gB of equine herpesvirus 4. Journal of Virology 63:1123–1133
     [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
     [Google Scholar]
  29. Schwartz R. M., Dayhoff M. O. 1978; Matrices for detecting distant relationship. Atlas of Protein Sequence and Structure 3:353–358
     [Google Scholar]
  30. Shimizu M., Satou K., Nishioka N. 1989; Monoclonal antibodies with neutralizing activity to equine herpesvirus 1. Archives of Virology 104:169–174
     [Google Scholar]
  31. Sinclair R., Cook R. F., Mumford J. A. 1989; The characterization of neutralizing and non-neutralizing monoclonal antibodies against equid herpesvirus type 1. Journal of General Virology 70:455–459
     [Google Scholar]
  32. Stokes A., Allen G. P., Pullen L. A., Murray P. K. 1989; A hamster model of equine herpesvirus type 1 (EHV-1) infection; passive protection by monoclonal antibodies to EHV-1 glycoproteins 13, 14 and 17/18. Journal of General Virology 70:1173–1183
     [Google Scholar]
  33. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 polymerase. Proceedings of the National Academy of Sciences, U.S.A 84:4767–4771
     [Google Scholar]
  34. Turtinen L. W., Allen G. P. 1982; Identification of the envelope surface glycoproteins of equine herpesvirus type 1. Journal of General Virology 63:481–485
     [Google Scholar]
  35. Wachsman M., Luo J. H., Aurelian L., Perkus M. E., Paoletti E. 1989; Antigen-presenting capacity of epidermal cells infected with vaccinia virus recombinants containing the herpes simplex virus glycoprotein D, and protective immunity. Journal of General Virology 70:2513–2520
     [Google Scholar]
  36. Watson R. J., Weis J. H., Salstrom J. S., EnQuist L. W. 1982; Herpes simplex virus type-1 glycoprotein D gene: nucleotide sequence and expression in Escherichia coli. Science 218:381–384
     [Google Scholar]
  37. Whalley J. M., Robertson G. R., Davison A. J. 1981; Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, BglII and BamHI. Journal of General Virology 57:307–323
     [Google Scholar]
  38. Whalley J. M., Robertson G. R., Scott N. A., Hudson G. C., Bell C. W., Woodworth L. M. 1989; Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB. Journal of General Virology 70:383–394 corrigendum 3513
    [Google Scholar]
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Equine Herpesvirus Type 1 Unique Short Fragment Encodes Glycoproteins with Homology to Herpes Simplex Virus Type 1 gD, gI and gE
J. Gen. Virol. 71, 2969 (1990); https://doi.org/10.1099/0022-1317-71-12-2969
/content/journal/jgv/10.1099/0022-1317-71-12-2969
/content/journal/jgv/10.1099/0022-1317-71-12-2969
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