1887

Abstract

To investigate the mechanisms of measles virus (MV) establishment and maintenance of persistence in lymphoid cells, we have established a long-term persistent infection with MV, Edmonston strain, in the human T lymphoblastoid cell line MOLT4, which has been in continuous culture for over 8 years. In this culture, designated MOMP1, more than 98% of cells display viral antigens. The MOMP1 culture is immune to superinfection with MV and is not cured by anti-MV antibodies. No evidence of defective interfering particles was obtained. The persistently infected culture releases an infectious virus showing a miniplaque and thermoresistant modified phenotype that, unlike the parental virus Edmonston strain which produces a lytic infection with extensive cell fusion, establishes an immediate persistence in MOLT4 cells with neither significant loss of cell viability nor cell fusion. This suggests that the modification in the virus suffices to maintain the state of persistence without requiring a coevolution of the host cell during the infection, as has been reported in other persistent virus infections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-9-2195
1992-09-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/9/JV0730092195.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-9-2195&mimeType=html&fmt=ahah

References

  1. Ahmed R., Canning W. M., Kauffman R. S., Sharpe A. H., Hallum J. V., Fields B. N. 1981; Role of the host cell in persistent viral infection: coevolution of L cell and reovirus during persistent infection. Cell 25:325–332
    [Google Scholar]
  2. Barry D. W., Sullivan J. L., Lucas S. J., Dunlap R. C., Albrecht T. 1976; Acute and chronic infection of human lymphoblastoid cell lines with measles virus. Journal of Immunology 116:89–98
    [Google Scholar]
  3. Calain P., Roux L. 1988; Generation of measles virus defective interfering particles and their presence in preparation of attenuated live-virus vaccine. Journal of Virology 62:2859–2866
    [Google Scholar]
  4. Celma M. L., Pan J., Weissman S. M. 1977; Studies of low molecular weight RNA from cells infected with adenovirus 2.I. The sequences at the 3′ end of VA-RNA I. Journal of Biological Chemistry 252:9032–9042
    [Google Scholar]
  5. de la Torre J. C., Martinez-Salas E., Diez J., Villaverde A., Gebauer F., Rocha E., Davila M., Domingo E. 1988; Coevolution of cell and viruses in a persistent infection of foot-and-mouth disease virus in cell culture. Journal of Virology 62:2050–2058
    [Google Scholar]
  6. Enami M., Kohama T., Sugiura A. 1989; A measles virus subgenomic RNA: structure and generation mechanism. Virology 171:427–439
    [Google Scholar]
  7. Fournier J. G., Tardieu M., Lebon P., Robain O., Ponsot G., Rozenblatt S., Bouteille M. 1985; Detection of measles virus RNA in lymphocytes from peripheral blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis. New England Journal of Medicine 313:910–915
    [Google Scholar]
  8. Fujinami R. S., Oldstone M. B. A. 1980; Alterations in expression of measles virus polypeptides by antibody: molecular events in antibody-induced antigen modulation. Journal of Immunology 125:78–85
    [Google Scholar]
  9. Gresser I., Chany C. 1963; Isolation of measles virus from washed leucocytic fraction of blood. Proceedings of the Society for Experimental Biology and Medicine 113:695–698
    [Google Scholar]
  10. Holland J. J., Kennedy S., Tan T., Semler B. L., Jones C. L., Roux L., Grabau E. A. 1980; Defective interfering RNA viruses and the host cell response. In Comprehensive Virology vol 16 pp 137–192 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  11. Horta-Barbosa L., Hamilton R., Witting B., Fucillo D. A., Sever J. L., Vernom M. L. 1971; Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science 173:840–841
    [Google Scholar]
  12. Jacobson S., McFarland H. F. 1982; Measles virus persistence in human lymphocytes: a role for virus-induced interferon. Journal of General Virology 63:351–357
    [Google Scholar]
  13. Joseph B. S., Lampert P. W., Oldstone M. B. A. 1975; Replication and persistence of measles virus in defined subpopulations of human lymphocytes. Journal of Virology 16:1638–1649
    [Google Scholar]
  14. Ju G., Udem S., Rager-Zisman B., Bloom B. R. 1978; Isolation of a heterogeneous population of ts mutants of measles virus from persistently infected human lymphoblastoid cell lines. Journal of Experimental Medicine 147:1637–1652
    [Google Scholar]
  15. Leppert M., Rittenhouse L., Perrault J., Summers D. F., Kolakofsky D. 1979; Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell 18:735–748
    [Google Scholar]
  16. McKimm-Breschkin J. L., Breschkin A. M., Rapp F. 1982; Characterization of the Halle SSPE measles virus isolate. Journal of General Virology 59:57–64
    [Google Scholar]
  17. Minowada J., Ohnuma T., Moore G. E. 1972; Rosette-forming human lymphoblastoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. Journal of the National Cancer Institute 49:891–895
    [Google Scholar]
  18. Norrby E., Oxman M. N. 1990; Measles. In Virology pp 1013–1044 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  19. Rager-Zisman B., Egan J. E., Kress Y., Bloom B. R. 1984; Isolation of cold sensitive mutants of measles virus from persistently infected murine lymphoblastoma cells. Journal of Virology 91:845–855
    [Google Scholar]
  20. Rima B. K., Davidson W. B., Martin S. J. 1977; The role of defective interfering particles in persistent infection of Vero cells by measles virus. Journal of General Virology 35:89–97
    [Google Scholar]
  21. Ron D., Tal J. 1985; Coevolution of cell and virus as a mechanism for the persistence of lymphotropic minute virus of mice in L cells. Journal of Virology 55:424–430
    [Google Scholar]
  22. Roux L., Holland J. J. 1979; Role of defective interfering particles of Sendai virus in persistent infections. Virology 93:91–103
    [Google Scholar]
  23. ter Meulen V., Stephenson J. R., Kreth H. W. 1983; Subacute sclerosing panencephalitis. In Comprehensive Virology vol 18 pp 105–159 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  24. Youngner J. S., Preble O. T. 1980; Viral persistence evolution of viral populations. In Comprehensive Virology vol 16 pp 73–135 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-9-2195
Loading
/content/journal/jgv/10.1099/0022-1317-73-9-2195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error