1887

Journal of General Virology header logo

Volume 73, Issue 9

Measles virus gene expression in lytic and persistent infections of a human lymphoblastoid cell line Free

Abstract

MOMP1 is a measles virus (MV) long-term steady-state persistently infected culture of the human T lymphoblastoid cell line MOLT4. The analysis of MV gene expression revealed that in MOMP1 cells, the major MV proteins, haemagglutinin (H), phosphoprotein (P), nucleoprotein, fusion (F) and matrix (M), are present and the fusion precursor (F) is cleaved into F and F peptides. H and F proteins are glycosylated in both lytic and persistent MOLT4 infections. All major proteins are underexpressed in the persistently infected cultures in comparison to the lytically infected cells. However a relatively greater reduction was observed for H, M and P proteins. Pulse-chase labelling experiments indicated that this underexpression of H, M and P proteins was not due to selective degradation of these proteins in the persistent infection (p.i.). The relative amounts of the major monocistronic and dicistronic mRNAs for MV proteins, with the exception of P mRNA, was not altered in the p.i. with respect to lytically infected MOLT4 cells, suggesting that the defective expression of H and M proteins was not due to a restriction in the transcription of their mRNAs. In contrast, the mRNA for P protein, the most abundant MV mRNA in these lytically infected T lymphoid cells, is markedly underexpressed in the homologous p.i. Thus the underexpression of P protein in p.i. could be due to a decreased availability of P mRNA. This unbalanced underexpression of MV proteins may impair the cell fusion and c.p.e. of MV and facilitate viral persistence in human lymphoid cells.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-9-2203
1992-09-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/9/JV0730092203.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-9-2203&mimeType=html&fmt=ahah

References

  1. Baczko K., Liebert U. G., Cattaneo R., Budka H., ter Meulen V. 1986; Expression of defective virus genes in brain tissue of patients with subacute sclerosing panencephalitis. Journal of Virology 59:472–478
     [Google Scholar]
  2. Baczko K., Liebert D. G., Cattaneo R., Billeter M. A., Roos R. P., ter Meulen V. 1988; Restriction of measles virus gene expression in measles inclusion body encephalitis. Journal of Infectious Diseases 158:144–150
     [Google Scholar]
  3. Barry D. W., Sullivan J. L., Lucas S. J., Dunlap R. C., Albrecht P. 1976; Acute and chronic infection of human lymphoblastoid cell lines with measles virus. Journal of Immunology 116:89–98
     [Google Scholar]
  4. Baslé M. F., Fournier J. G., Rozenblatt S., Rebel A., Bouteille M. 1986; Measles virus RNA detected in Paget’s disease bone tissue by in situ hybridization. Journal of General Virology 67:907–913
     [Google Scholar]
  5. Carter M. J., Willcocks M. M., Loffler S., ter Meulen V. 1982; Relationships between monoclonal antibody-binding sites on measles virus haemagglutinin. Journal of General Virology 63:113–120
     [Google Scholar]
  6. Cattaneo R., Rebman G., Schmid A., Baczko K., ter Meulen V., Billeter M. 1987a; Altered transcription of a defective measles virus genome derived from a diseased human brain. EMBO Journal 6:681–688
     [Google Scholar]
  7. Cattaneo R., Rebman G., Baczko K., ter Meulen V., Billeter M. A. 1987b; Altered ratios of measles virus transcripts in diseased human brains. Virology 160:523–526
     [Google Scholar]
  8. Cattaneo R., Schmid A., Spielhofer P., Kaelin K., Baczko K., ter Meulen V., Pardowitz J., Flanagan S., Rima B., Udem S., Billeter M. A. 1989; Mutated and hypermutated genes of persistent measles virus which cause lethal human brain diseases. Virology 173:415–425
     [Google Scholar]
  9. Fernandez-Muñoz R., Celma M. L. 1992; Measles virus from a long-term persistently infected human T lymphoblastoid cell line, in contrast to the cytocidal parental virus, establishes an immediate persistence in the original cell line. Journal of General Virology 73:2195–2202
     [Google Scholar]
  10. Fournier J. G., Tardieu M., Lebon P., Robain O., Ponsot G., Rozenblatt S., Bouteille M. 1985; Detection of measles virus RNA in lymphocytes from peripheral blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis. New England Journal of Medicine 313:910–915
     [Google Scholar]
  11. Francis S. J., Southern P. J. 1988; Molecular analysis of viral RNAs in the mice persistently infected with lymphocytic choriomeningitis virus. Journal of Virology 62:1251–1257
     [Google Scholar]
  12. Graves M. C. 1981; Measles virus polypeptides in infected cells studied by immune precipitation and one-dimensional peptide mapping. Journal of Virology 38:224–230
     [Google Scholar]
  13. Gresser I., Chany C. 1963; Isolation of measles virus from the washed leucocyte fraction of blood. Proceedings of the Society for Experimental Biology 113:695–698
     [Google Scholar]
  14. Haase A. T., Gantz D., Eble B., Walker D., Stowrig L., Ventura P., Blum H., Wietgriefe S., Zupancic M., Tourtel-lote W., Gibbs C. J., Norrby E., Rozenblatt S. 1985; Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosising panencephalitis. Proceedings of the National Academy of Sciences, U.S.A. 82:3020–3024
     [Google Scholar]
  15. Hall W., Choppin P. W. 1981; Measles virus proteins in the brain tissue of patients with subacute sclerosing panencephalitis. New England Journal of Medicine 304:1152–1155
     [Google Scholar]
  16. Horta-Barbosa L., Hamilton R., Witting B., Fucillo D. A., Sever J. L., Vernom M. L. 1971; Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science 173:840–841
     [Google Scholar]
  17. Joseph B. S., Lampert P. W., Oldstone M. B. A. 1975; Replication and persistence of measles virus in defined populations of human leukocytes. Journal of Virology 16:1638–1649
     [Google Scholar]
  18. Ju G., Udem S., Rager-Zisman B., Bloom B. R. 1978; Isolation of a heterogeneous population of ts mutants of measles virus from persistently infected human lymphoblastoid cell lines. Journal of Experimental Medicine 147:1637–1652
     [Google Scholar]
  19. Kristensson K., Norrby E. 1986; Persistence of RNA viruses in the central nervous system. Annual Review of Microbiology 40:159–184
     [Google Scholar]
  20. Leppert M., Rittenhouse L., Perrault J., Summers D. F., Kolakofsky D. 1979; Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell 18:735–748
     [Google Scholar]
  21. Liebert U. G., Baczko K., Budka H., ter Meulen V. 1986; Restricted expression of measles virus proteins in brains from cases of subacute sclerosing panencephalitis. Journal of General Virology 67:2435–2444
     [Google Scholar]
  22. Moench T. R., Griffin D. E., Obriecht C. R., Vaisberg A. J., Johnson R. T. 1988; Acute measles in patients with and without neurological involvement: distribution of measles virus antigen and RNA. Journal of Infectious Diseases 158:433–442
     [Google Scholar]
  23. Norrby E., Chen S. N., Togashi T., Sheshberadaran H., Johnson K. P. 1982; Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Archives of Virology 71:1–11
     [Google Scholar]
  24. Norrby E., Kristensson K., Brzosko W. J., Kapsenberg J. G. 1985; Measles virus matrix protein detected by immune fluorescence with monoclonal antibodies in the brain of patients with subacute sclerosing panencephalitis. Journal of Virology 56:337–340
     [Google Scholar]
  25. Oldstone M. B. A. 1989; Viral persistence. Cell 56:517–520
     [Google Scholar]
  26. Oldstone M. B. A., Buchmeier M. J. 1982; Restricted expression of viral glycoprotein in cells of persistently infected mice. Nature, London 300:360–362
     [Google Scholar]
  27. Roux L., Waldvogel F. A. 1982; Instability of the viral M protein in BHK-21 cells persistently infected with Sendai virus. Cell 28:293–302
     [Google Scholar]
  28. Rupp R. A., Weintraub H. 1991; Ubiquitous Myo D transcription at the midbiastula: transcription precedes induction-dependent Myo D expression in presumptive mesoderm of X. laevis . Cell 65:927–937
     [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  30. Sheppard R. D., Raine C. S., Bornstein M. B., Udem S. A. 1986; Rapid degradation restricts measles virus matrix protein expression in a subacute sclerosing panencephalitis cell line. Proceedings of the National Academy of Sciences, U.S.A. 83:7913–7917
     [Google Scholar]
  31. Singer-Sam J., Robinson M. O., Bellve A. R., Simon M. I., Riggs A. D. 1990; Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase and Zly gene transcripts during mouse spermatogenesis. Nucleic Acids Research 18:1255–1259
     [Google Scholar]
  32. ter Meulen V., Carter M. J. 1984; Measles virus persistence and disease. Progress in Medical Virology 30:44–61
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-9-2203
Loading
Measles virus gene expression in lytic and persistent infections of a human lymphoblastoid cell line
J. Gen. Virol. 73, 2203 (1992); https://doi.org/10.1099/0022-1317-73-9-2203
/content/journal/jgv/10.1099/0022-1317-73-9-2203
/content/journal/jgv/10.1099/0022-1317-73-9-2203
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error