1887

Abstract

Respiratory syncytial virus (RSV) has been isolated from sheep suffering from respiratory tract disease. Since the greatest differences between bovine RSV and human RSV are found on the attachment G protein, we have determined the nucleotide and deduced amino acid sequences of the G gene of ovine RSV. The latter contained 838 nucleotides and had a major open reading frame encoding a protein of 263 residues, and shared 73% nucleotide sequence identity with that of bovine RSV. The deduced amino acid sequence of the ovine RSV G protein showed only 60% amino acid identity with the G protein of bovine RSV. Despite the low level of identity, there were similarities in the predicted hydropathy profiles of the G proteins of ovine and bovine RSV. The intergenic sequences for the SH–G and G–F gene junctions of ovine RSV showed 64 and 57% identity respectively with the corresponding regions of the bovine RSV. Our results indicate that ovine and bovine RSV might be classified as two subgroups of an ungulate RSV.

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1993-12-01
2024-05-18
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References

  1. Anderson L. J., Heirholzer J. C., Tsou C., Hendry R. M., Fernie B. N., Stone Y., McIntosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  2. Berthiaume L., Joncase J., Boulay G., Pavilanis V. 1973; Serological evidence of respiratory syncytial virus infection in sheep. Veterinary Record 89:337–338
    [Google Scholar]
  3. Cane P. A., Matthews D. A., Pringle C. R. 1991; Identification of variable domains of the attachment (G) protein of subgroup A respiratory syncytial viruses. Journal of General Virology 72:2091–2096
    [Google Scholar]
  4. Cutlip R. C., Lehmkuhl H. D. 1979; Lesions in lambs experimentally infected with bovine respiratory syncytial virus. American Journal of Veterinary Research 40:1479–1482
    [Google Scholar]
  5. Evermann J. F., Liggitt H. D., Parish S. M., Ward A. C. S., Leamaster B. R. 1985; Properties of a respiratory syncytial virus isolated from a sheep with rhinitis. American Journal of Veterinary Research 46:947–951
    [Google Scholar]
  6. Gimenez H. B., Hardman N., Keir H. M., Cash P. 1986; Antigenic variation between human respiratory syncytial virus isolates. Journal of General Virology 67:863–870
    [Google Scholar]
  7. Huang Y. T., Collins P. L., Wertz G. W. 1985; Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. Virus Research 2:157–173
    [Google Scholar]
  8. Johnson P. R., Collins P. L. 1988; The A and B subgroups of human respiratory syncytial virus: comparison of intergenic and gene-overlap sequences. Journal of General Virology 69:2901–2906
    [Google Scholar]
  9. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987; The G glycoprotein of human respiratory syncytial virus of subgroup A and B: extensive sequence divergence between anti-genically related proteins. Proceedings of the National Academy of Sciences, U.S.A. 84:5625–5629
    [Google Scholar]
  10. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  11. Lehmkuhl H. D., Smith M. H., Cutlip R. C. 1980; Morphogenesis and structure of caprine respiratory syncytial virus. Archives of Virology 65:269–279
    [Google Scholar]
  12. Lerch R. A., Stott E. J., Wertz G. W. 1989; Characterization of bovine respiratory syncytial virus proteins and mRNAs and generation of cDNA clones to the viral mRNAs. Journal of Virology 63:833–840
    [Google Scholar]
  13. Lerch R. A., Anderson K., Wertz G. W. 1990; Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. Journal of Virology 64:5559–5569
    [Google Scholar]
  14. Levine S., Klaiber-Franco R., Paradiso P. R. 1987; Demonstration that glycoprotein G is the attachment protein of respiratory syncytial virus. Journal of General Virology 68:2521–2524
    [Google Scholar]
  15. Mallipeddi S. K., Samal S. K. 1992; Sequence comparison between the phosphoprotein mRNAs of human and bovine respiratory syncytial viruses identifies a divergent domain in the predicted protein. Journal of General Virology 73:2441–2444
    [Google Scholar]
  16. Mallipeddi S. K., Samal S. K. 1993; Sequence variability of the glycoprotein gene of bovine respiratory syncytial virus. Journal of General Virology 74:2001–2004
    [Google Scholar]
  17. Mufson M. A., Orvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  18. Myers E. W., Miller W. 1988; Optimal alignments in linear space. Computer Applications in the Biosciences 4:11–17
    [Google Scholar]
  19. Samal S. K., Zamora M. 1991; Nucleotide sequence analysis of a matrix and small hydrophobic protein dicistronic mRNA of bovine respiratory syncytial virus demonstrates extensive sequence divergence of the small hydrophobic protein from that of human respiratory syncytial virus. Journal of General Virology 72:1715–1720
    [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  21. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. 1985; Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Research 13:7795–7812
    [Google Scholar]
  22. Spraker T. R., Collins J. K. 1986; Isolation and serological evidence of a respiratory syncytial virus in bighorn sheep from Colorado. Journal of Wildlife Diseases 22:416–418
    [Google Scholar]
  23. Stott E. J., Taylor G. 1985; Respiratory syncytial virus: brief review. Archives of Virology 84:1–52
    [Google Scholar]
  24. Sullender W. M., Mufson M. A., Anderson L. J., Wertz G. 1991; Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses. Journal of Virology 65:5425–5434
    [Google Scholar]
  25. Walravens K., Kettmann R., Collard A., Coppe P., Burny A. 1990; Sequence comparison between the fusion protein of human and bovine respiratory syncytial viruses. Journal of General Virology 71:3009–3014
    [Google Scholar]
  26. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. 1985; Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, U.S.A 82:4075–4079
    [Google Scholar]
  27. Zamora M., Samal S. K. 1992; Gene junction sequences of bovine respiratory syncytial virus. Virus Research 24:115–121
    [Google Scholar]
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