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Volume 78, Issue 9

Infectious cDNA clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the disease Free

Abstract

A full-length infectious cDNA clone of strawberry mild yellow edge-associated potexvirus (SMYEaV) was constructed and used to inoculate ‘Alpine’ seedlings and . Both host plants could be infected using particle bombardment or agroinoculation, but not by mechanical inoculation. A method that used potted strawberry plants for particle bombardment resulted in high survival and infection rates. The plants developed systemic infection and virus particles were detected by ELISA and immuno-electron microscopy. Mechanical inoculation of and with the 35S construct resulted in localized infections. ‘Alpine’ indicator plants produced symptoms that were indistinguishable from control plants inoculated with a naturally occurring isolate of strawberry mild yellow edge by graft or aphid transmission. These results suggest that SMYE potexvirus is the causal agent of strawberry mild yellow edge disease. As this virus is capable of causing the disease, we propose the name strawberry mild yellow edge potexvirus, with the acronym SMYEPV, to replace the name strawberry mild yellow edge-associated potexvirus.

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© Society for General Microbiology Ltd 1997
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1997-09-01
2024-04-26
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Infectious cDNA clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the disease
J. Gen. Virol. 78, 2347 (1997); https://doi.org/10.1099/0022-1317-78-9-2347
/content/journal/jgv/10.1099/0022-1317-78-9-2347
/content/journal/jgv/10.1099/0022-1317-78-9-2347
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