1887

Journal of General Virology header logo

Volume 81, Issue 7

Multiple infection, recombination and genome relationships among begomovirus isolates found in cotton and other plants in Pakistan Free

Abstract

Begomoviruses occur in many plant species in Pakistan and are associated with an epidemic of cotton leaf curl disease that has developed since 1985. PCR analysis with primer pairs specific for each of four already sequenced types of DNA-A of cotton leaf curl virus (CLCuV-PK types a, 26, 72b and 804a), or for okra yellow vein mosaic virus (OYVMV), indicated that many individual naturally infected plants of cotton and other malvaceous species contained two or three begomovirus sequences. Similarly, sequence differences among overlapping fragments of begomovirus DNA-A, amplified from individual naturally infected plants, indicated much multiple infection in malvaceous and non-malvaceous species. Some cotton plants contained DNA-A sequences typical of begomoviruses from non-malvaceous species, and some non-malvaceous plants contained sequences typical of CLCuV-PK. Some DNA-A sequences were chimaeric; they each included elements typical of different types of CLCuV-PK, or of different malvaceous and/or non-malvaceous begomoviruses. Often an apparent recombination site occurred at the origin of replication. No complete CLCuV-PK DNA-A sequence was found in malvaceous or non-malvaceous species collected in Pakistan outside the area of the cotton leaf curl epidemic but chimaeric sequences, including a part that was typical of CLCuV-PK DNA-A, did occur there. We suggest that recombination among such pre-existing sequences was crucial for the emergence of CLCuV-PK. Recombination, following multiple infection, could also explain the network of relationships among many of the begomoviruses found in the Indian subcontinent, and their evolutionary divergence, as a group, from begomoviruses causing similar diseases in other geographical regions.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-7-1839
2000-07-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/7/0811839a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-7-1839&mimeType=html&fmt=ahah

References

  1. Ali, M., Ahmad, Z., Tanveer, M. & Mahmood, T. (1995).Cotton Leaf Curl Virus in the Punjab: Current Situation and Review of Work. Multan: Central Cotton Research Institute/Ministry of Food, Agriculture and Livestock, Government of Pakistan/Asian Development Bank.
  2. Anon. (1994). Program Manual for the Wisconsin Package, Version 8. Madison: Genetics Computer Group.
  3. Arguello-Astorga, G., Herrera-Estrella, L. & Rivera-Bustamante, R. (1994). Experimental and theoretical definition of geminivirus origin of replication. Plant Molecular Biology 26, 553-556.[CrossRef] [Google Scholar]
  4. Brown, J. K. & Bird, J. (1992). Whitefly-transmitted geminiviruses and associated disorders in the Americas and the Caribbean Basin. Plant Disease 76, 220-225.[CrossRef] [Google Scholar]
  5. Brown, J. K., Frolich, D. R. & Rosell, R. C. (1995). The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology 40, 511-534.[CrossRef] [Google Scholar]
  6. Fontes, E. P. B., Gladfelter, H. J., Schaffer, R. L., Petty, I. T. D. & Hanley-Bowdoin, L. (1994). Geminivirus replication origins have a modular organization. Plant Cell 6, 405-416.[CrossRef] [Google Scholar]
  7. Frischmuth, T., Roberts, S., von Arnim, A. & Stanley, J. (1993). Specificity of bipartite geminivirus movement proteins. Virology 196, 666-673.[CrossRef] [Google Scholar]
  8. Harrison, B. D. (1985). Advances in geminivirus research. Annual Review of Phytopathology 23, 55-82.[CrossRef] [Google Scholar]
  9. Harrison, B. D. & Robinson, D. J. (1999). Natural genomic and antigenic variation in whitefly-transmitted geminiviruses (begomoviruses). Annual Review of Phytopathology 37, 369-398.[CrossRef] [Google Scholar]
  10. Harrison, B. D., Liu, Y. L., Khalid, S., Hameed, S., Otim-Nape, G. W. & Robinson, D. J. (1997a). Detection and relationships of cotton leaf curl virus and allied whitefly-transmitted geminiviruses occurring in Pakistan. Annals of Applied Biology 130, 61-75.[CrossRef] [Google Scholar]
  11. Harrison, B. D., Zhou, X., Otim-Nape, G. W., Liu, Y. & Robinson, D. J. (1997b). Role of a novel type of double infection in the geminivirus-induced epidemic of severe cassava mosaic in Uganda. Annals of Applied Biology 131, 437-448.[CrossRef] [Google Scholar]
  12. Hou, Y.-M., Paplomatas, E. J. & Gilbertson, R. L. (1998). Host adaptation and replication properties of two bipartite geminiviruses and their pseudorecombinants. Molecular Plant–Microbe Interactions 11, 208-217.[CrossRef] [Google Scholar]
  13. Ingham, D. J., Pascal, E. & Lazarowitz, S. (1995). Both bipartite geminivirus movement proteins define viral host range, but only BLI determines viral pathogenicity. Virology 207, 191-204.[CrossRef] [Google Scholar]
  14. Jupin, I., Hericourt, F., Benz, B. & Gronenborn, B. (1995). DNA replication specificity of TYLCV geminivirus is mediated by the amino-terminal 116 amino acids of the Rep protein. FEBS Letters 362, 116-120.[CrossRef] [Google Scholar]
  15. Lazarowitz, S. G. (1991). Molecular characterization of two bipartite geminiviruses causing squash leaf curl disease: role of viral replication and movement functions in determining host range. Virology 180, 70-80.[CrossRef] [Google Scholar]
  16. Lazarowitz, S. G., Wu, L. C., Rogers, S. G. & Elmer, J. S. (1992). Sequence-specific interaction with the viral ALI protein identifies a geminivirus DNA replication origin. Plant Cell 4, 799-809.[CrossRef] [Google Scholar]
  17. Liu, Y., Robinson, D. J. & Harrison, B. D. (1998). Defective forms of cotton leaf curl virus DNA-A that have different combinations of sequence deletion, duplication, inversion and rearrangement. Journal of General Virology 79, 1501-1508. [Google Scholar]
  18. Mansoor, S., Khan, S. H., Bashir, A., Saeed, M., Zafar, Y., Malik, K. A., Briddon, R., Stanley, J. & Markham, P. G. (1999). Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 259, 190-199.[CrossRef] [Google Scholar]
  19. Nadeem, A. (1995).Molecular characterization of two cotton geminiviruses. PhD Dissertation, University of Arizona, USA.
  20. Nateshan, H. M., Muniyappa, V., Swanson, M. M. & Harrison, B. D. (1996). Host range, vector relations and serological relationships of cotton leaf curl virus from southern India. Annals of Applied Biology 128, 233-244.[CrossRef] [Google Scholar]
  21. Navot, N., Pichersky, E., Zeidan, M., Zamir, D. & Csoznek, H. (1991). Tomato yellow leaf curl virus; a whitefly-transmitted geminivirus with a single genomic molecule. Virology 185, 151-161.[CrossRef] [Google Scholar]
  22. Padidam, M., Beachy, R. N. & Fauquet, C. M. (1995a). Tomato leaf curl geminivirus in India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology 76, 25-35.[CrossRef] [Google Scholar]
  23. Padidam, M., Beachy, R. N. & Fauquet, C. M. (1995b). Classification and identification of geminiviruses using sequence comparisons. Journal of General Virology 76, 249-263.[CrossRef] [Google Scholar]
  24. Sanz, A. I., Fraile, A., Gallego, J. M., Malpica, J. M. & Garcı́a-Arenal, F. (1999). Genetic variability of natural populations of cotton leaf curl geminivirus, a single-stranded DNA virus. Journal of Molecular Evolution 49, 672-681.[CrossRef] [Google Scholar]
  25. Stanley, J. (1995). Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nonanucleotide motif during the initiation of rolling circle DNA replication. Virology 206, 707-712.[CrossRef] [Google Scholar]
  26. Swanson, M. M., Varma, A., Muniyappa, V. & Harrison, B. D. (1992). Comparative epitope profiles of the particle proteins of whitefly-transmitted geminiviruses from nine crop legumes in India. Annals of Applied Biology 120, 425-433.[CrossRef] [Google Scholar]
  27. Zhou, X., Liu, Y., Robinson, D. J. & Harrison, B. D. (1998). Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra. Journal of General Virology 79, 915-923. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-7-1839
Loading
Multiple infection, recombination and genome relationships among begomovirus isolates found in cotton and other plants in Pakistan
J. Gen. Virol. 81, 1839 (2000); https://doi.org/10.1099/0022-1317-81-7-1839
/content/journal/jgv/10.1099/0022-1317-81-7-1839
/content/journal/jgv/10.1099/0022-1317-81-7-1839
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error