1887

Journal of General Virology header logo

Volume 99, Issue 7

Prevalence and genome characteristics of canine astrovirus in southwest China Free

Abstract

The aim of this study was to investigate canine astrovirus (CaAstV) infection in southwest China. We collected 107 faecal samples from domestic dogs with obvious diarrhoea. Forty-two diarrhoeic samples (39.3 %) were positive for CaAstV by RT-PCR, and 41/42 samples showed co-infection with canine coronavirus (CCoV), canine parvovirus-2 (CPV-2) and canine distemper virus (CDV). Phylogenetic analysis based on 26 CaAstV partial ORF1a and ORF1b sequences revealed that most CaAstV strains showed unique evolutionary features. Interestingly, putative recombination events were observed among four of the five complete ORF2 sequences cloned in this study, and three of the five complete ORF2 sequences formed a single unique group, suggesting that these strains could be a novel genotype. We successfully sequenced the complete genome of one CaAstV strain (designated 2017/44/CHN), which was 6628 nt in length. The features of this genome include putative recombination events in the ORF1a, ORF1b and ORF2 genes, while the ORF2 gene had a continuous insertion of 7 aa in region II compared with the other complete ORF2 sequences available in GenBank. Phylogenetic analysis showed that 2017/44/CHN formed a single group based on genome sequences, suggesting that this strain might be a novel genotype. The results of this study revealed that CaAstV circulates widely in diarrhoeic dogs in southwest China and exhibits unique evolutionary events. To the best of our knowledge, this is the first report of recombination events in CaAstV, and it contributes to further understanding of the genetic evolution of CaAstV.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Canine astrovirus , genome , molecular characteristics , prevalence and recombination
© 2018 The Authors
Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001077
2018-07-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/99/7/880.html?itemId=/content/journal/jgv/10.1099/jgv.0.001077&mimeType=html&fmt=ahah

References

  1. Williams FP. Astrovirus-like, coronavirus-like, and parvovirus-like particles detected in the diarrheal stools of beagle pups. Arch Virol 1980; 66:215–226 [View Article][PubMed]
     [Google Scholar]
  2. Toffan A, Jonassen CM, De Battisti C, Schiavon E, Kofstad T et al. Genetic characterization of a new astrovirus detected in dogs suffering from diarrhoea. Vet Microbiol 2009; 139:147–152 [View Article][PubMed]
     [Google Scholar]
  3. Martella V, Moschidou P, Catella C, Larocca V, Pinto P et al. Enteric disease in dogs naturally infected by a novel canine astrovirus. J Clin Microbiol 2012; 50:1066–1069 [View Article][PubMed]
     [Google Scholar]
  4. Choi S, Lim SI, Kim YK, Cho YY, Song JY et al. Phylogenetic analysis of astrovirus and kobuvirus in Korean dogs. J Vet Med Sci 2014; 76:1141–1145 [View Article][PubMed]
     [Google Scholar]
  5. Lizasoain A, Tort LF, García M, Gómez MM, Leite JP et al. Sewage surveillance reveals the presence of canine GVII norovirus and canine astrovirus in Uruguay. Arch Virol 2015; 160:2839–2843 [View Article][PubMed]
     [Google Scholar]
  6. Zhou H, Liu L, Li R, Qin Y, Fang Q et al. Detection and genetic characterization of canine astroviruses in pet dogs in Guangxi, China. Virol J 2017; 14:156 [View Article][PubMed]
     [Google Scholar]
  7. Grellet A, de Battisti C, Feugier A, Pantile M, Marciano S et al. Prevalence and risk factors of astrovirus infection in puppies from French breeding kennels. Vet Microbiol 2012; 157:214–219 [View Article][PubMed]
     [Google Scholar]
  8. Martella V, Moschidou P, Lorusso E, Mari V, Camero M et al. Detection and characterization of canine astroviruses. J Gen Virol 2011; 92:1880–1887 [View Article][PubMed]
     [Google Scholar]
  9. Takano T, Takashina M, Doki T, Hohdatsu T. Detection of canine astrovirus in dogs with diarrhea in Japan. Arch Virol 2015; 160:1549–1553 [View Article][PubMed]
     [Google Scholar]
  10. Caddy SL, Goodfellow I. Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains. Vet Microbiol 2015; 177:206–213 [View Article][PubMed]
     [Google Scholar]
  11. Mihalov-Kovács E, Martella V, Lanave G, Bodnar L, Fehér E et al. Genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission. Virus Res 2017; 232:162–170 [View Article][PubMed]
     [Google Scholar]
  12. Moreno PS, Wagner J, Mansfield CS, Stevens M, Gilkerson JR et al. Characterisation of the canine faecal virome in healthy dogs and dogs with acute diarrhoea using shotgun metagenomics. PLoS One 2017; 12:e0178433 [View Article][PubMed]
     [Google Scholar]
  13. Zhu AL, Zhao W, Yin H, Shan TL, Zhu CX et al. Isolation and characterization of canine astrovirus in China. Arch Virol 2011; 156:1671–1675 [View Article][PubMed]
     [Google Scholar]
  14. Lukashov VV, Goudsmit J. Evolutionary relationships among Astroviridae. J Gen Virol 2002; 83:1397–1405 [View Article][PubMed]
     [Google Scholar]
  15. Arias CF, Dubois RM. The astrovirus capsid: a review. Viruses 2017; 9:15 [View Article][PubMed]
     [Google Scholar]
  16. de Grazia S, Medici MC, Pinto P, Moschidou P, Tummolo F et al. Genetic heterogeneity and recombination in human type 2 astroviruses. J Clin Microbiol 2012; 50:3760–3764 [View Article][PubMed]
     [Google Scholar]
  17. Babkin IV, Tikunov AY, Sedelnikova DA, Zhirakovskaia EV, Tikunova NV. Recombination analysis based on the HAstV-2 and HAstV-4 complete genomes. Infect Genet Evol 2014; 22:94–102 [View Article][PubMed]
     [Google Scholar]
  18. Ha HJ, Lee SG, Cho HG, Jin JY, Lee JW et al. Complete genome sequencing of a recombinant strain between human astrovirus antigen types 2 and 8 isolated from South Korea. Infect Genet Evol 2016; 39:127–131 [View Article][PubMed]
     [Google Scholar]
  19. Bull RA, Hansman GS, Clancy LE, Tanaka MM, Rawlinson WD et al. Norovirus recombination in ORF1/ORF2 overlap. Emerg Infect Dis 2005; 11:1079–1085 [View Article][PubMed]
     [Google Scholar]
  20. Martella V, Medici MC, Terio V, Catella C, Bozzo G et al. Lineage diversification and recombination in type-4 human astroviruses. Infect Genet Evol 2013; 20:330–335 [View Article][PubMed]
     [Google Scholar]
  21. Wolfaardt M, Kiulia NM, Mwenda JM, Taylor MB. Evidence of a recombinant wild-type human astrovirus strain from a Kenyan child with gastroenteritis. J Clin Microbiol 2011; 49:728–731 [View Article][PubMed]
     [Google Scholar]
  22. Pativada MS, Chatterjee D, Mariyappa NS, Rajendran K, Bhattacharya MK et al. Emergence of unique variants and inter-genotype recombinants of human astroviruses infecting infants, children and adults in Kolkata, India. Int J Mol Epidemiol Genet 2011; 2:228–235[PubMed]
     [Google Scholar]
  23. Bull RA, Tanaka MM, White PA. Norovirus recombination. J Gen Virol 2007; 88:3347–3359 [View Article][PubMed]
     [Google Scholar]
  24. Donato C, Vijaykrishna D. The broad host range and genetic diversity of mammalian and avian astroviruses. Viruses 2017; 9:102 [View Article][PubMed]
     [Google Scholar]
  25. Verma H, Chitambar SD, Gopalkrishna V. Astrovirus associated acute gastroenteritis in western India: predominance of dual serotype strains. Infect Genet Evol 2010; 10:575–579 [View Article][PubMed]
     [Google Scholar]
  26. Van Hemert FJ, Lukashov VV, Berkhout B. Different rates of (non-) synonymous mutations in astrovirus genes; correlation with gene function. Virol J 2007; 4:25–12 [View Article][PubMed]
     [Google Scholar]
  27. Decaro N, Elia G, Martella V, Desario C, Campolo M et al. A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs. Vet Microbiol 2005; 105:19–28 [View Article][PubMed]
     [Google Scholar]
  28. Elia G, Decaro N, Martella V, Cirone F, Lucente MS et al. Detection of canine distemper virus in dogs by real-time RT-PCR. J Virol Methods 2006; 136:171–176 [View Article][PubMed]
     [Google Scholar]
  29. Decaro N, Pratelli A, Campolo M, Elia G, Martella V et al. Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR. J Virol Methods 2004; 119:145–150 [View Article][PubMed]
     [Google Scholar]
  30. Martin D, Rybicki E. RDP: detection of recombination amongst aligned sequences. Bioinformatics 2000; 16:562–563 [View Article][PubMed]
     [Google Scholar]
  31. Kosakovsky Pond SL, Posada D, Gravenor MB, Woelk CH, Frost SD. GARD: a genetic algorithm for recombination detection. Bioinformatics 2006; 22:3096–3098 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001077
Loading
Prevalence and genome characteristics of canine astrovirus in southwest China
J. Gen. Virol. 99, 880 (2018); https://doi.org/10.1099/jgv.0.001077
/content/journal/jgv/10.1099/jgv.0.001077
/content/journal/jgv/10.1099/jgv.0.001077
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error