1887

Abstract

JC virus (JCV) DNA replication occurs in the nuclei of infected cells. The level of JCV genome expression depends on nucleotide sequences in the viral regulatory region and their interaction with host-cell nuclear transcription factors. Our previous studies showed a higher level of NF-1X in JCV-permissive cells compared with the other members of the NF-1 family, NF-1A, B and C, which suggests that NF-1X plays a positive role in JCV multiplication. It remained unclear whether a reduction in the level of NF-1A, which is expressed abundantly in JCV-non-permissive cell types, leads to an increase in JCV multiplication. In this study, we show that downregulation of NF-1A expression in JCV-non-susceptible progenitor and HeLa cells results in a reversion to susceptibility for JCV multiplication. These data demonstrate that a higher level of NF-1A protein in JCV-non-permissive cell types, compared with the level of NF-1X, may be acting as a negative regulator at the JCV promoter to control JCV multiplication.

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2008-06-01
2024-04-27
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References

  1. Amemiya K., Traub R., Durham L., Major E. O. 1989; Interaction of a nuclear factor-1-like protein with the regulatory region of the human polyomavirus JC virus. J Biol Chem 264:7025–7032
    [Google Scholar]
  2. Amemiya K., Traub R., Durham L., Major E. O. 1992; Adjacent nuclear factor-1 and activator protein binding sites in the enhancer of the neurotropic JC virus. A common characteristic of many brain-specific genes. J Biol Chem 267:14204–14211
    [Google Scholar]
  3. Astrom K. E., Mancall E. L., Richardson E. P. 1958; Progressive multifocal leukoencephalopathy; a hitherto unrecognized complication of chronic lymphatic leukaemia and Hodgkin's disease. Brain 81:93–127 [CrossRef]
    [Google Scholar]
  4. Berger J. R., Major E. O. 1999; Progressive multifocal leukoencephalopathy. Semin Neurol 19:193–200 [CrossRef]
    [Google Scholar]
  5. Bisgrove D. A., Monckton E. A., Packer M., Godbout R. 2000; Regulation of brain fatty acid-binding protein expression by differential phosphorylation of nuclear factor I in malignant glioma cell lines. J Biol Chem 275:30668–30676 [CrossRef]
    [Google Scholar]
  6. Chen N. N., Khalili K. 1995; Transcriptional regulation of human JC polyomavirus promoters by cellular proteins YB-1 and Pur alpha in glial cells. J Virol 69:5843–5848
    [Google Scholar]
  7. Ciappi S., Azzi A., De Santis R., Leoncini F., Sterrantino G., Mazzotta F., Mecocci L. 1999; Archetypal and rearranged sequences of human polyomavirus JC transcription control region in peripheral blood leukocytes and in cerebrospinal fluid. J Gen Virol 80:1017–1023
    [Google Scholar]
  8. Delbue S., Sotgiu G., Fumagalli D., Valli M., Borghi E., Mancuso R., Marchioni E., Maserati R., Ferrante P. 2005; A case of a progressive multifocal leukoencephalopathy patient with four different JC virus transcriptional control region rearrangements in cerebrospinal fluid, blood, serum, and urine. J Neurovirol 11:51–57 [CrossRef]
    [Google Scholar]
  9. Fazi F., Rosa A., Fatica A., Gelmetti V., De Marchis M. L., Nervi C., Bozzoni I. 2005; A minicircuitry comprised of microRNA-223 and transcription factors NFI-A and C/EBPalpha regulates human granulopoiesis. Cell 123:819–831 [CrossRef]
    [Google Scholar]
  10. Gronostajski R. M. 2000; Roles of the NFI/CTF gene family in transcription and development. Gene 249:31–45 [CrossRef]
    [Google Scholar]
  11. Hamilton R. S., Gravell M., Major E. O. 2000; Comparison of antibody titers determined by hemagglutination inhibition and enzyme immunoassay for JC virus and BK virus. J Clin Microbiol 38:105–109
    [Google Scholar]
  12. Imperiale M. J., Major E. O. 2007; Polyomaviruses. In Field's Virology , 5th edn. pp 2263–2298Edited by Knipe D. M., Howley P. M., Griffin D. E., Lamb R. A., Straus S. E., Martin M. A., Roizman. New York: Lippincott, Williams & Wilkins;
    [Google Scholar]
  13. Jeang K. T., Rawlins D. R., Rosenfeld P. J., Shero J. H., Kelly T. J., Hayward G. S. 1987; Multiple tandemly repeated binding sites for cellular nuclear factor 1 that surround the major immediate-early promoters of simian and human cytomegalovirus. J Virol 61:1559–1570
    [Google Scholar]
  14. Jones K. A., Kadonaga J. T., Rosenfeld P. J., Kelly T. J., Tjian R. 1987; A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. Cell 48:79–89 [CrossRef]
    [Google Scholar]
  15. Kleene R., Yang H., Kutsche M., Schachner M. 2001; The neural recognition molecule L1 is a sialic acid-binding lectin for CD24, which induces promotion and inhibition of neurite outgrowth. J Biol Chem 276:21656–21663 [CrossRef]
    [Google Scholar]
  16. Kruse U., Sippel A. E. 1994; Transcription factor nuclear factor I proteins form stable homo- and heterodimers. FEBS Lett 348:46–50 [CrossRef]
    [Google Scholar]
  17. Kruse U., Qian F., Sippel A. E. 1991; Identification of a fourth nuclear factor I gene in chicken by cDNA cloning: NFI-X. Nucleic Acids Res 19:6641 [CrossRef]
    [Google Scholar]
  18. Messam C. A., Major E. O. 2000; Stages of restricted HIV-1 infection in astrocyte cultures derived from human fetal brain tissue. J Neurovirol 6:Suppl. 1S90–S94
    [Google Scholar]
  19. Messam C. A., Hou J., Gronostajski R. M., Major E. O. 2003; Lineage pathway of human brain progenitor cells identified by JC virus susceptibility. Ann Neurol 53:636–646 [CrossRef]
    [Google Scholar]
  20. Nathanson N., Ahmed R., Gonzalez-Scarano F., Griffin D. E., Holmes K., Murphy F., Robinson H. editors 1997 Viral Pathogenesis New York: Lippincott–Raven;
    [Google Scholar]
  21. Raj G. V., Khalili K. 1995; Transcriptional regulation: lessons from the human neurotropic polyomavirus, JCV. Virology 213:283–291 [CrossRef]
    [Google Scholar]
  22. Ravichandran V., Sabath B. F., Jensen P. N., Houff S. A., Major E. O. 2006; Interactions between c-Jun, nuclear factor 1, and JC virus promoter sequences: implications for viral tropism. J Virol 80:10506–10513 [CrossRef]
    [Google Scholar]
  23. Ravichandran V., Jensen P. N., Major E. O. 2007; MEK1/2 inhibitors block basal and transforming growth factor 1beta1-stimulated JC virus multiplication. J Virol 81:6412–6418 [CrossRef]
    [Google Scholar]
  24. Shaul Y., Ben-Levy R., De-Medina T. 1986; High affinity binding site for nuclear factor I next to the hepatitis B virus S gene promoter. EMBO J 5:1967–1971
    [Google Scholar]
  25. Sumner C., Shinohara T., Durham L., Traub R., Major E. O., Amemiya K. 1996; Expression of multiple classes of the nuclear factor-1 family in the developing human brain: differential expression of two classes of NF-1 genes. J Neurovirol 2:87–100 [CrossRef]
    [Google Scholar]
  26. Tamura T., Inoue T., Nagata K., Mikoshiba K. 1988; Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts. Biochem Biophys Res Commun 157:419–425 [CrossRef]
    [Google Scholar]
  27. Walker D. L., Padgett B. L. 1983; The epidemiology of human polyomaviruses. Prog Clin Biol Res 105:99–106
    [Google Scholar]
  28. Wong Y. W., Schulze C., Streichert T., Gronostajski R. M., Schachner M., Tilling T. 2007; Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation. Genome Biol 8:R72 [CrossRef]
    [Google Scholar]
  29. Yousry T. A., Major E. O., Ryschkewitsch C., Fahle G., Fischer S., Hou J., Curfman B., Miszkiel K., Mueller-Lenke N. other authors 2006; Evaluation of patients treated with natalizumab for progressive multifocal leukoencephalopathy. N Engl J Med 354:924–933 [CrossRef]
    [Google Scholar]
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