1887

Journal of General Virology header logo

Volume 89, Issue 12

Examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines Free

Abstract

Comparisons of the relative activities of 11 intergenic region (IGR) internal ribosome entry site (IRES) elements of insect with 5′ IRES elements of the hepatitis C and encephalomyocarditis viruses were performed in insect and mammalian cells. Dual luciferase assays were performed to determine the most effective dicistrovirus IGR IRES in the lepidopteran cell lines Sf9 () and BmN (), and the dipteran cell lines S2 () and ATC-10 (). Evaluation of dual luciferase expression from DNA plasmids and -transcribed RNA revealed apparent splicing with certain IRES elements. Though IRES activity depended upon the cell line examined, the black queen cell and C dicistrovirus intergenic IRES elements were most effective for coupled gene expression in the diverse insect cell lines examined.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/003921-0
2008-12-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/12/3150.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/003921-0&mimeType=html&fmt=ahah

References

  1. Argaud O., Croizier L., Lopez-Ferber M., Croizier G. 1998; Two key mutations in the host-range specificity domain of the p143 gene of Autographa californica nucleopolyhedrovirus are required to kill Bombyx mori larvae. J Gen Virol 79:931–935
     [Google Scholar]
  2. Baird S. D., Turcotte M., Korneluk R. G., Holcik M. 2006; Searching for IRES. RNA 12:1755–1785 [CrossRef]
     [Google Scholar]
  3. Bieleski L., Talbot S. J. 2001; Kaposi's sarcoma-associated herpesvirus vCyclin open reading frame contains an internal ribosome entry site. J Virol 75:1864–1869 [CrossRef]
     [Google Scholar]
  4. Blaney J. E. Jr, Matro J. M., Murphy B. R., Whitehead S. S. 2005; Recombinant, live-attenuated tetravalent dengue virus vaccine formulations induce a balanced, broad, and protective neutralizing antibody response against each of the four serotypes in rhesus monkeys. J Virol 79:5516–5528 [CrossRef]
     [Google Scholar]
  5. Borman A. M., Kirchweger R., Ziegler E., Rhoads R. E., Skern T., Kean K. M. 1997; elF4G and its proteolytic cleavage products: effect on initiation of protein synthesis from capped, uncapped, and IRES-containing mRNAs. RNA 3:186–196
     [Google Scholar]
  6. Cohen J., Charpilienne A., Chilmonczyk S., Estes M. K. 1989; Nucleotide sequence of bovine rotavirus gene 1 and expression of the gene product in baculovirus. Virology 171:131–140 [CrossRef]
     [Google Scholar]
  7. Grace T. D. 1967; Establishment of a line of cells from the silkworm Bombyx mori . Nature 216:613
     [Google Scholar]
  8. Grasela J. J., McIntosh A. H., Goodman C. L., Wilson L. E., King L. A. 2000; Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines. In Vitro Cell Dev Biol Anim 36205–210 [CrossRef]
     [Google Scholar]
  9. Grundhoff A., Ganem D. 2001; Mechanisms governing expression of the v-FLIP gene of Kaposi's sarcoma-associated herpesvirus. J Virol 75:1857–1863 [CrossRef]
     [Google Scholar]
  10. Hellen C. U., Sarnow P. 2001; Internal ribosome entry sites in eukaryotic mRNA molecules. Genes Dev 15:1593–1612 [CrossRef]
     [Google Scholar]
  11. Herbreteau C. H., Weill L., Decimo D., Prevot D., Darlix J. L., Sargueil B., Ohlmann T. 2005; HIV-2 genomic RNA contains a novel type of IRES located downstream of its initiation codon. Nat Struct Mol Biol 12:1001–1007 [CrossRef]
     [Google Scholar]
  12. Holcik M., Korneluk R. G. 2000; Functional characterization of the X-linked inhibitor of Apoptosis (XIAP) internal ribosome entry site element: role of La autoantigen in XIAP translation. Mol Cell Biol 20:4648–4657 [CrossRef]
     [Google Scholar]
  13. Honda M., Kaneko S., Matsushita E., Kobayashi K., Abell G. A., Lemon S. M. 2000; Cell cycle regulation of hepatitis C virus internal ribosomal entry site-directed translation. Gastroenterology 118:152–162 [CrossRef]
     [Google Scholar]
  14. Jan E. 2006; Divergent IRES elements in invertebrates. Virus Res 119:16–28 [CrossRef]
     [Google Scholar]
  15. Johnson A. D., Krieg P. A. 1995; A Xenopus laevis gene encoding EF-1 alpha S, the somatic form of elongation factor 1 alpha: sequence, structure, and identification of regulatory elements required for embryonic transcription. Dev Genet 17:280–290 [CrossRef]
     [Google Scholar]
  16. Koch H., Friesen J. D. 1979; Individual messenger RNA half lives in Saccharomyces cerevisiae . Mol Gen Genet 170:129–135 [CrossRef]
     [Google Scholar]
  17. Lerat H., Shimizu Y. K., Lemon S. M. 2000; Cell type-specific enhancement of hepatitis C virus internal ribosome entry site-directed translation due to 5′ nontranslated region substitutions selected during passage of virus in lymphoblastoid cells. J Virol 74:7024–7031 [CrossRef]
     [Google Scholar]
  18. Mange A., Julien E., Prudhomme J. C., Couble P. 1997; A strong inhibitory element down-regulates SRE-stimulated transcription of the A3 cytoplasmic actin gene of Bombyx mori . J Mol Biol 265:266–274 [CrossRef]
     [Google Scholar]
  19. Masoumi A., Hanzlik T. N., Christian P. D. 2003; Functionality of the 5′- and intergenic IRES elements of cricket paralysis virus in a range of insect cell lines, and its relationship with viral activities. Virus Res 94:113–120 [CrossRef]
     [Google Scholar]
  20. Matera A. G., Terns R. M., Terns M. P. 2007; Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs. Nat Rev Mol Cell Biol 8:209–220 [CrossRef]
     [Google Scholar]
  21. Mayo M. A. 2002; A summary of taxonomic changes recently approved by ICTV. Arch Virol 147:1655–1663 [CrossRef]
     [Google Scholar]
  22. McIntosh A. H., Grasela J. J., Popham H. J. 2005; AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda . In Vitro Cell Dev Biol Anim 41298–304 [CrossRef]
     [Google Scholar]
  23. Parsons S. J., Rhodes S. A., Connor H. E., Rees S., Brown J., Giles H. 2000; Use of a dual firefly and Renilla luciferase reporter gene assay to simultaneously determine drug selectivity at human corticotrophin releasing hormone 1 and 2 receptors. Anal Biochem 281:187–192 [CrossRef]
     [Google Scholar]
  24. Pear W. S., Nolan G. P., Scott M. L., Baltimore D. 1993; Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A 90:8392–8396 [CrossRef]
     [Google Scholar]
  25. Roberts L. O., Seamons R. A., Belsham G. J. 1998; Recognition of picornavirus internal ribosome entry sites within cells; influence of cellular and viral proteins. RNA 4:520–529 [CrossRef]
     [Google Scholar]
  26. Sasaki J., Nakashima N. 1999; Translation initiation at the CUU codon is mediated by the internal ribosome entry site of an insect picorna-like virus in vitro . J Virol 73:1219–1226
     [Google Scholar]
  27. Schneider I. 1972; Cell lines derived from late embryonic stages of Drosophila melanogaster . J Embryol Exp Morphol 27:353–365
     [Google Scholar]
  28. Shibuya N., Nakashima N. 2006; Characterization of the 5′ internal ribosome entry site of Plautia stali intestine virus. J Gen Virol 87:3679–3686 [CrossRef]
     [Google Scholar]
  29. Shibuya N., Nishiyama T., Kanamori Y., Saito H., Nakashima N. 2003; Conditional rather than absolute requirements of the capsid coding sequence for initiation of methionine-independent translation in Plautia stali intestine virus. J Virol 77:12002–12010 [CrossRef]
     [Google Scholar]
  30. Singh K. R., Pavri K. M. 1967; Experimental studies with chikungunya virus in Aedes aegypti and Aedes albopictus . Acta Virol 11:517–526
     [Google Scholar]
  31. Stables J., Scott S., Brown S., Roelant C., Burns D., Lee M. G., Rees S. 1999; Development of a dual glow-signal firefly and Renilla luciferase assay reagent for the analysis of G-protein coupled receptor signalling. J Recept Signal Transduct Res 19:395–410 [CrossRef]
     [Google Scholar]
  32. Thoetkiattikul H., Beck M. H., Strand M. R. 2005; Inhibitor κ B-like proteins from a polydnavirus inhibit NF- κ B activation and suppress the insect immune response. Proc Natl Acad Sci U S A 102:11426–11431 [CrossRef]
     [Google Scholar]
  33. Van Eden M. E., Byrd M. P., Sherrill K. W., Lloyd R. E. 2004; Demonstrating internal ribosome entry sites in eukaryotic mRNAs using stringent RNA test procedures. RNA 10:720–730 [CrossRef]
     [Google Scholar]
  34. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda ( Lepidoptera ; Noctuidae ). In Vitro 13213–217 [CrossRef]
     [Google Scholar]
  35. Weiss I. M., Liebhaber S. A. 1994; Erythroid cell-specific determinants of alpha-globin mRNA stability. Mol Cell Biol 14:8123–8132
     [Google Scholar]
  36. Weiss I. M., Liebhaber S. A. 1995; Erythroid cell-specific mRNA stability elements in the alpha 2-globin 3′ nontranslated region. Mol Cell Biol 15:2457–2465
     [Google Scholar]
  37. Wilson J. E., Powell M. J., Hoover S. E., Sarnow P. 2000; Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites. Mol Cell Biol 20:4990–4999 [CrossRef]
     [Google Scholar]
  38. Woolaway K. E., Lazaridis K., Belsham G. J., Carter M. J., Roberts L. O. 2001; The 5′ untranslated region of Rhopalosiphum padi virus contains an internal ribosome entry site which functions efficiently in mammalian, plant, and insect translation systems. J Virol 75:10244–10249 [CrossRef]
     [Google Scholar]
  39. Yamamura K., Miki T., Suzuki N., Ebihara T., Kawai K., Kumahara Y., Honjo T. 1985; Introduction of mouse Cϵ genes into Cos-7 cells and fertilized mouse eggs. J Biochem 97:333–339
     [Google Scholar]
  40. Zhao Y. G., Eggleston P. 1999; Comparative analysis of promoters for transient gene expression in cultured mosquito cells. Insect Mol Biol 8:31–38 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/003921-0
Loading
Examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines
J. Gen. Virol. 89, 3150 (2008); https://doi.org/10.1099/vir.0.2008/003921-0
/content/journal/jgv/10.1099/vir.0.2008/003921-0
/content/journal/jgv/10.1099/vir.0.2008/003921-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error